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Different PES nanofibrous membrane parameters effect on the efficacy of immunoassay performance

Mahmoudifard, M ; Sharif University of Technology | 2019

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  1. Type of Document: Article
  2. DOI: 10.1002/pat.4629
  3. Publisher: John Wiley and Sons Ltd , 2019
  4. Abstract:
  5. The contribution of electrospun nanofibrous membranes (e.NFMs) in the biosensing platforms opens up a new prospect for the invention of faster and more sensitive analytical devices. In this paper, we utilized e.NFM of polyethersulfone (PES) as a solid substrate for the protein immobilization through two different approaches: physical and covalent. Scanning electron microscopy (SEM) and Fourier-transform-infrared (FTIR) tests were performed to study the effect of plasma treatment on protein immobilization efficacy. Moreover, taking advantage of ELISA technique, the influence of different parameters, namely, nanofibers diameter, membrane thickness, plasma treatment time, an incubation time of ethyl-3-(3-dimethylaminopropyl)-carbodiimide/N-hydroxysuccinimide (EDC/NHS), and their ratio on antibody immobilization efficacy through two mentioned approaches, was also assessed. © 2019 John Wiley & Sons, Ltd
  6. Keywords:
  7. Biosensor nanofiber ; Plasma modification ; Fourier transform infrared spectroscopy ; Plasma applications ; Proteins ; Scanning electron microscopy ; Antibody immobilization ; Biosensing platforms ; Electrospun nanofibrous membranes ; Fourier transform infrared ; Membrane thickness ; Nanofibrous membranes ; Plasma modifications ; Protein immobilization ; Nanofibers ; Electrospinning
  8. Source: Polymers for Advanced Technologies ; Volume 30, Issue 8 , 2019 , Pages 1968-1977 ; 10427147 (ISSN)
  9. URL: https://onlinelibrary.wiley.com/doi/abs/10.1002/pat.4629