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antibody
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VEGF Isolation from Platelet Lysate
, M.Sc. Thesis Sharif University of Technology ; Abdekhodaie, Mohammad Jafar (Supervisor)
Abstract
Platelet-rich plasma as a juvenile to the narrow repertoire of orthopedic medicine, has risen hope to attain a generic autologous regenerative formula for different conditions. Vast plethora of GFs, cytokines and chemokines has endowed PRP with excellent regenerative properties, however not all of them would favor different conditions. Vascular endothelial growth factor has been demonstrated to be involved in the progress of Osteoarthritis by amplifying the catabolic processes which deteriorate the extracellular matrix of cartilage. Though cumbersome, the result of eliminating VEGF from platelet lysate would be promising. VEGF isolation from platelet lysate requires selectivity. By employing...
Label-Free real-time detection of HBsAg using a QCM immunosensor
, Article Clinical Laboratory ; Volume 68, Issue 4 , 2022 , Pages 707-720 ; 14336510 (ISSN) ; Ghavidel, A ; Ahangari Cohan, R ; Hamidi Fard, M ; Khoobi, M ; Aghasadeghi, M ; Norouzian, D ; Sharif University of Technology
Verlag Klinisches Labor GmbH
2022
Abstract
Background: Hepatitis B virus surface antigen (HBsAg) is an important protein in both diagnosis and prevention of hepatitis B infection. In the current study, a piezoelectric immunosensor based on antibody-antigen interaction was designed to detect HBsAg. A quartz crystal microbalance system was employed to detect antibody-antigen interaction. Methods: At first, an oscillator was designed to measure the resonant frequency affected by the reactants using IC 74LVC1GX04. Antibody against HBsAg was immobilized on 10 MHz AT-cut quartz crystal. The surface modifications were monitored by atomic force microscopy (AFM) and contact angle measurements. Different concentrations of antibody were used...
Design of an effective piezoelectric microcantilever biosensor for rapid detection of COVID-19
, Article Journal of Medical Engineering and Technology ; Volume 45, Issue 6 , 2021 , Pages 423-433 ; 03091902 (ISSN) ; Merati, M ; Abdekhodaie, M. J ; Sharif University of Technology
Taylor and Francis Ltd
2021
Abstract
Acute respiratory syndrome coronavirus 2 (SARS-CoV-2), also called COVID-19, is one of the most contagious viruses resulting in a progressive pandemic. Since specific antiviral treatments have not been developed yet and its fatal rate is almost high, early and fast detection is critical for controlling the outbreak. In this study, a piezoelectric microcantilever biosensor has been designed for detecting COVID-19 samples directly without requiring preparation steps. The biosensor acts as a transducer and is coated with the related antibody. When the SARS-CoV-2 antigens adsorbed on the microcantilever top surface through their spike proteins, a surface stress due to the mass change would be...
Electrospray: novel fabrication method for biodegradable polymeric nanoparticles for further applications in drug delivery systems
, Article NANOCON 2009 - 1st International Conference, Conference Proceedings ; 2009 , Pages 324-331 ; 9788087294130 (ISBN) ; Vossoughi, M ; Sharif University of Technology
TANGER Ltd
2009
Abstract
Electrospray is an old processing technique that has recently been rediscovered. This technology is revolutionizing the biomaterials and nanotechnology fields by opening up new areas of research in fabricating drug delivery nano-devices. Due to their size, nanoparticles have the advantage of reaching less accessible sites in the body by escaping phagocytosis and entering tiny capillaries. Nanoparticles also have the unique property to accumulate at the site of inflammation and therefore, are very suitable for targeted drug delivery. The feasibility of fabricating relatively monodispersed polymeric nanoparticles by electrospray method in a modified electrospray setup is demonstrated in this...
Personalised deposition maps for micro- and nanoparticles targeting an atherosclerotic plaque: attributions to the receptor-mediated adsorption on the inflamed endothelial cells
, Article Biomechanics and Modeling in Mechanobiology ; Volume 18, Issue 3 , 2019 , Pages 813-828 ; 16177959 (ISSN) ; Forouzandehmehr, M ; Sharif University of Technology
Springer Verlag
2019
Abstract
Endothelial inflammation as a prominent precursor to atherosclerosis elicits a distinct pathological surface expression of particular vascular proteins. To exhibit a site-specific behaviour, micro- and nanoparticles, as carriers of therapeutics or imaging agents, can distinguish and use these proteins as targeted docking sites. Here, a computational patient-specific model capturing the exclusive luminal qualities has been developed to study the transport and adsorption of particles decorated with proper antibodies over an atherosclerotic plaque located in the LAD artery of the patient. Particles, in nano- and micron sizes, have been decorated with Sialyl Lewisx (sLex), P-selectin aptamer...
Serological assays and host antibody detection in coronavirus-related disease diagnosis
, Article Archives of Virology ; Volume 166, Issue 3 , 2021 , Pages 715-731 ; 03048608 (ISSN) ; Shabani, E ; Abdekhodaie, M. J ; Sharif University of Technology
Springer
2021
Abstract
Coronaviruses (CoV) are a family of viral pathogens that infect both birds and mammals, including humans. Seven human coronaviruses (HCoV) have been recognized so far. HCoV-229E, -OC43, -NL63, and -HKU1 account for one-third of common colds with mild symptoms. The other three members are severe acute respiratory syndrome (SARS)-CoV, Middle East respiratory syndrome (MERS)-CoV, and SARS-CoV-2. These viruses are responsible for SARS, MERS, and CoV disease 2019 (COVID-19), respectively. A variety of diagnostic techniques, including chest X-rays, computer tomography (CT) scans, analysis of viral nucleic acids, proteins, or whole virions, and host antibody detection using serological assays have...
Dual improvement of DNA-directed antibody immobilization utilizing magnetic fishing and a polyamine coated surface
, Article RSC Advances ; Volume 6, Issue 112 , 2016 , Pages 111210-111216 ; 20462069 (ISSN) ; Soleimani, M ; Shamloo, A ; Mahmoudifard, M ; Vossoughi, M ; Sharif University of Technology
Royal Society of Chemistry
2016
Abstract
The present study is aimed at the development of a novel approach based on the magnetic improvement of DNA-directed antibody immobilization to prepare a highly efficient sensing platform. Magnetic nanoparticle substrates with high surface area capture the dual DNA-conjugated antibodies in a solution. This allows overcoming the typical mass transport limitation of the surface-based antibody immobilization. Antibody-magnetic nanoparticle conjugation is based on a robust hybridization between a DNA tether (attached to the antibody) and its complementary sequence (immobilized on the nanoparticle). Conventional antibody immobilization for the detection of proteins is often insignificant for the...
Designed amino acid feed in improvement of production and quality targets of a therapeutic monoclonal antibody
, Article PLoS ONE ; Volume 10, Issue 10 , October , 2015 ; 19326203 (ISSN) ; Vaziri, B ; Maleknia, S ; Heydari, A ; Vossoughi, M ; Davami, F ; Mahboudi, F ; Sharif University of Technology
Public Library of Science
2015
Abstract
Cell culture feeds optimization is a critical step in process development of pharmaceutical recombinant protein production. Amino acids are the basic supplements of mammalian cell culture feeds with known effect on their growth promotion and productivity. In this study, we reported the implementation of the Plackett-Burman (PB) multifactorial design to screen the effects of amino acids on the growth promotion and productivity of a Chinese hamster ovary DG-44 (CHO-DG44) cell line producing bevacizumab. After this screening, the amino acid combinations were optimized by the response surface methodology (RSM) to determine the most effective concentration in feeds. Through this strategy, the...
Design and development of a self-assembling protein nanoparticle displaying PfHAP2 antigenic determinants recognized by natural acquired antibodies
, Article PLoS ONE ; Volume 17, Issue 9 September , 2022 ; 19326203 (ISSN) ; Mehrizi, A. A ; Sardari, S ; Alemzadeh, I ; Sharif University of Technology
Public Library of Science
2022
Abstract
Backgrounds In order to move towards the elimination and eradication of malaria in the world, the development of vaccines is inevitable. Many modern vaccines are based on recombinant technology; however, they may not provide a fully protective, long-lasting immune response. One of the strategies to improve recombinant vaccines is designing the nanovaccines such as self-assembling protein nanoparticles (SAPNs). Hence, the presentation of epitopes in a repeat array and correct conformation should be considered. P. falciparum generative cell-specific 1 (PfGCS1) is a main transmission-blocking vaccine candidate with two highly conserved fragments, HAP2-GCS1 and cd loop, inducing partial malaria...
Quantitative proteomic analysis of cellular responses to a designed amino acid feed in a monoclonal antibody producing Chinese hamster ovary cell line
, Article Iranian Biomedical Journal ; Volume 22, Issue 6 , 2018 , Pages 385-393 ; 1028852X (ISSN) ; Mahboudi, F ; Vossoughi, M ; Fatemi, E ; Moosavi Basri, S. M ; Heydari, A ; Vaziri, B ; Sharif University of Technology
Pasteur Institute of Iran
2018
Abstract
Background: Chinese hamster ovary (CHO) cell line is considered as the most common cell line in the biopharmaceutical industry because of its capability in performing efficient post-translational modifications and producing the recombinant proteins, which are similar to natural human proteins. The optimization of the upstream process via different feed strategies has a great impact on the target molecule expression and yield. Methods: To determine and understand the molecular events beneath the feed effects on the CHO cell, a label-free quantitative proteomic analysis was applied. The proteome changes followed by the addition of a designed amino acid feed to the monoclonal antibody producing...
Point-of-use rapid detection of sars-cov-2: Nanotechnology-enabled solutions for the covid-19 pandemic
, Article International Journal of Molecular Sciences ; Volume 21, Issue 14 , 2020 , Pages 1-23 ; Bagherzadeh, M ; Ghasemi, A ; Zare, H ; Ahmadi, S ; Fatahi, Y ; Dinarvand, R ; Rabiee, M ; Ramakrishna, S ; Shokouhimehr, M ; Varma, R. S ; Sharif University of Technology
MDPI AG
2020
Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) caused the COVID-19 pandemic that has been spreading around the world since December 2019. More than 10 million affected cases and more than half a million deaths have been reported so far, while no vaccine is yet available as a treatment. Considering the global healthcare urgency, several techniques, including whole genome sequencing and computed tomography imaging have been employed for diagnosing infected people. Considerable efforts are also directed at detecting and preventing different modes of community transmission. Among them is the rapid detection of virus presence on different surfaces with which people may come in...
Dna-Rna hybrid (R-loop): From a unified picture of the mammalian telomere to the genome-wide profile
, Article Cells ; Volume 10, Issue 6 , 2021 ; 20734409 (ISSN) ; Sharifi Zarchi, A ; Kianmehr, L ; Sharif University of Technology
MDPI
2021
Abstract
Local three-stranded DNA/RNA hybrid regions of genomes (R-loops) have been detected either by binding of a monoclonal antibody (DRIP assay) or by enzymatic recognition by RNaseH. Such a structure has been postulated for mouse and human telomeres, clearly suggested by the identification of the complementary RNA Telomeric repeat-containing RNA “TERRA”. However, the tremendous disparity in the information obtained with antibody-based technology drove us to investigate a new strategy. Based on the observation that DNA/RNA hybrids in a triplex complex genome co-purify with the double-stranded chromosomal DNA fraction, we developed a direct preparative approach from total protein-free cellular...
Enzymatic formation of recombinant antibody-conjugated gold nanoparticles in the presence of citrate groups and bacteria
, Article Catalysts ; Volume 12, Issue 9 , 2022 ; 20734344 (ISSN) ; Ebrahimipour, G ; Bandehpour, M ; Akhavan, O ; Yarian, F ; Sharif University of Technology
MDPI
2022
Abstract
With the spread of deadly diseases worldwide, the design of rapid tests to identify causative microorganisms is necessary. Due to the unique properties of gold nanoparticles, these nanoparticles are used in designing rapid diagnostic tests, such as strip tests. The current study aimed to investigate the ability of gold nanoparticles to bind to single-chain variable fragment antibodies. In this study, the biological and chemical methods included Escherichia coli TOP-10 and the Turkevich method to synthesize the gold nanoparticles, respectively. Then, the effect of synthetic nanoparticles on their capability of binding to recombinant antibodies was assessed by agarose gel and UV-vis...
Multilayered mesoporous composite nanostructures for highly sensitive label-free quantification of cardiac troponin-i
, Article Biosensors ; Volume 12, Issue 5 , 2022 ; 20796374 (ISSN) ; Amidian, M. A ; Sheybanikashani, S ; Mahdavi, H ; Alimohammadi, H ; Syedmoradi, L ; Mohandes, F ; Zarrabi, A ; Tamjid, E ; Omidfar, K ; Simchi, A ; Sharif University of Technology
MDPI
2022
Abstract
Cardiac troponin-I (cTnI) is a well-known biomarker for the diagnosis and control of acute myocardial infarction in clinical practice. To improve the accuracy and reliability of cTnI electrochemical immunosensors, we propose a multilayer nanostructure consisting of Fe3O4-COOH labeled anti-cTnI monoclonal antibody (Fe3O4-COOH-Ab1 ) and anti-cTnI polyclonal antibody (Ab2 ) conjugated on Au-Ag nanoparticles (NPs) decorated on a metal–organic framework (Au-Ag@ZIF-67-Ab2 ). In this design, Fe3O4-COOH was used for separation of cTnI in specimens and signal amplification, hierarchical porous ZIF-67 extremely enhanced the specific surface area, and Au-Ag NPs synergically promoted the conductivity...
A noninvasive urine metabolome panel as potential biomarkers for diagnosis of t cell-mediated renal transplant rejection
, Article OMICS A Journal of Integrative Biology ; Volume 24, Issue 3 , March , 2020 , Pages 140-147 ; Chashmniam, S ; Nafar, M ; Samavat, S ; Rezaie, D ; Dalili, N ; Sharif University of Technology
Mary Ann Liebert Inc
2020
Abstract
Acute T cell-mediated rejection (TCMR)is a major complication after renal transplantation. TCMR diagnosis is very challenging and currently depends on invasive renal biopsy and nonspecific markers such as serum creatinine. A noninvasive metabolomics panel could allow early diagnosis and improved accuracy and specificity. We report, in this study, on urine metabolome changes in renal transplant recipients diagnosed with TCMR, with a view to future metabolomics-based diagnostics in transplant medicine. We performed urine metabolomic analyses in three study groups: (1) 7 kidney transplant recipients with acute TCMR, (2) 15 kidney transplant recipients without rejection but with impaired kidney...
Different PES nanofibrous membrane parameters effect on the efficacy of immunoassay performance
, Article Polymers for Advanced Technologies ; Volume 30, Issue 8 , 2019 , Pages 1968-1977 ; 10427147 (ISSN) ; Vossoughi, M ; Sharif University of Technology
John Wiley and Sons Ltd
2019
Abstract
The contribution of electrospun nanofibrous membranes (e.NFMs) in the biosensing platforms opens up a new prospect for the invention of faster and more sensitive analytical devices. In this paper, we utilized e.NFM of polyethersulfone (PES) as a solid substrate for the protein immobilization through two different approaches: physical and covalent. Scanning electron microscopy (SEM) and Fourier-transform-infrared (FTIR) tests were performed to study the effect of plasma treatment on protein immobilization efficacy. Moreover, taking advantage of ELISA technique, the influence of different parameters, namely, nanofibers diameter, membrane thickness, plasma treatment time, an incubation time of...
Electrospun polyethersolfone nanofibrous membrane as novel platform for protein immobilization in microfluidic systems
, Article Journal of Biomedical Materials Research - Part B Applied Biomaterials ; Volume 106, Issue 3 , April , 2018 , Pages 1108-1120 ; 15524973 (ISSN) ; Vossoughi, M ; Soudi, S ; Soleimani, M ; Sharif University of Technology
John Wiley and Sons Inc
2018
Abstract
In the present study, the feasibility of electrospun polyethersolfone (PES) nanofibrous membrane as the solid substrate for microfluidic based immunoassays to enhance the density of immobilized antibody on the surface of membrane was assessed. Conversely, the efficacy of antibody immobilization was compared by two different strategies as 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/N-Hydroxysuccinimide (NHS) coupling chemistry and hydrophobic interaction. Compared to conventional immunoassays carried out in plates or gels, microfluidic based immunoassays grant a lot of advantages such as a consumption of little samples and reagents, shorter analysis time, and higher efficiency....
HLA alleles and haplotype frequencies in Iranian population
, Article Human Antibodies ; Volume 30, Issue 2 , 2022 , Pages 79-96 ; 10932607 (ISSN) ; Hussen, B. M ; Pashmforoush, S ; Akbari, M. T ; Arsang-Jang, S ; Nazer, N ; Hamidieh, A. A ; Hajifathali, A ; Dinger, M. E ; Sayad, A ; Dehaghi, M. O ; Sharif University of Technology
IOS Press BV
2022
Abstract
BACKGROUND: HLA genotyping is a prerequisite for selection of suitable donors in the process of bone marrow transplantation. METHODS: In the current study, the frequencies of HLA-A, -B, -C and -DRB1 alleles and A-B-C-DRB1 haplotypes were assessed in 855 healthy Iranian persons using a low-resolution sequence specific primer (SSP) kit. RESULTS: Frequencies were compared between 11 subpopulations including Armani, Balouch, Bandari, Turk, Turkaman, Arab, Fars, Kurd, Gilaki, Lor and Mazani. In total, 17 HLA-A alleles were detected, one of which (HLA-A*74) was present only among Lors. HLA-A*23 and -A*26 were the most frequent HLA-A alleles among Armanis. HLA-A*23 was also common among Turkamans....
Development of a nano biosensor for anti-gliadin detection for Celiac disease based on suspension microarrays
, Article Biomedical Physics and Engineering Express ; Volume 6, Issue 5 , August , 2020 ; Rabiee, M ; Rostami Nejad, M ; Aghamohammadi, E ; Asadzadeh Aghdaei, H ; Zali, M. R ; Rabiee, N ; Fatahi, Y ; Bagherzadeh, M ; Webster, T. J ; Sharif University of Technology
IOP Publishing Ltd
2020
Abstract
Celiac disease is an autoimmune disorder represented by the ingestion of the gluten protein usually found in wheat, barley and rye. To date, ELISA has been the most accurate method for determining the presence of anti-gliadin, which is cumbersome, expensive (compared to a suspension microarray technique), and requires extensive sample preparation. In this study, in order to establish a more accurate assay to identify gliadin at lower concentrations, optical nano biosensors using an indirect immunoassay method for gliadin detection was designed and fabricated. For this, polycaprolactone (PCL) nano- to micro-beads were fabricated as a platform for the gliadin antigen which were optimized and...
Efficient protein immobilization on polyethersolfone electrospun nanofibrous membrane via covalent binding for biosensing applications
, Article Materials Science and Engineering C ; Volume 58 , 2016 , Pages 586-594 ; 09284931 (ISSN) ; Soudi, S ; Soleimani, M ; Hosseinzadeh, S ; Esmaeili, E ; Vossoughi, M ; Sharif University of Technology
Elsevier Ltd
2016
Abstract
In this paper we introduce novel strategy for antibody immobilization using high surface area electrospun nanofibrous membrane based on ethyl-3-(3-dimethylaminopropyl)-carbodiimide/N-hydroxysuccinimide (EDC/NHS) coupling chemistry. To present the high performance of proposed biosensors, anti-staphylococcus enterotoxin B (anti-SEB) was used as a model to demonstrate the utility of our proposed system. Polymer solution of polyethersolfone was used to fabricate fine nanofibrous membrane. Moreover, industrial polyvinylidene fluoride membrane and conventional microtiter plate were also used to compare the efficiency of antibody immobilization. Scanning electron microscopy images were taken to...