Investigating the Effect of Hemoglobin on Viscoelastic Properties of RBC

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Felekary, Arash | 2018

1777 Viewed

Type of Document: M.Sc. Thesis
Language: Farsi
Document No: 51032 (04)
University: Sharif University of Technology
Department: Physics
Advisor(s): Seyed Reihani, Nader
Abstract:
Red blood cells are one of the most important parts of blood and their mechanical properties are so important in some diseases. Red blood cell is mostly consisted of membrane, cell skeleton, and cytoplasm which contains hemoglobin proteins. Studying these properties separately not only could give us pieces of information about cell mechanics, but also reveals a way to predict their behavior after illnesses. Optical tweezers as a tool, which can produce and measure Piconewton to Nanonewton forces, has been interesting in red blood cell parts' studying. In this thesis, at first hemoglobin is taken out gradually by using Saponin (which is enable to make some pores in the membrane) and the exited amount of it is measured by analysis of spectrum. At second experiment, cells treated by Saponin were exposured to tension by optical tweezers and their viscoelastic properties were examined as well. The results indicate that by reducing the amount of hemoglobin, the stiffness of red blood cells increases. Besides, treated RBCs with Saponin follows the linear viscoelasticity after pores being made.This could have been taken place since ATP, the metabolism factor in cells, comes out of membrane
Keywords:
Hemoglobin ; Red Blood Cell ; Viscoelasticity ; Saponin ; Optical Tweezer ; Viscoelastic Behavior