Sharif Digital Repository

Blood is the most important liquid in human body. Red blood cells (RBCs) are the main part of blood. RBCs are rounded, but this shape would be changed during different disease. Blood tests taken to diagnose blood diseases. Current instruments do not provide any information about RBCs morphology, and an expert recognize the RBCs morphology by looking at blood smear under microscope. Regards to low accuracy and performance of this method, an automate method must be presented. Capturing images from blood smear under microscope is simple and low-cost. Although there is few methods on RBCs detection and very limited one on RBCs recognition on the literature, no general method to recognize most of... 

Red blood cells (RBC, erythrocytes) are cells with an average diameter of 8 microns, the thickness of 2-3 microns and the volume of 90 fl. The biconcave shape of red blood cells,together with cell membrane flexibility, enables them to pass the capillaries whose diameter is considerably less than 8 micrometers. Mechanical properties of RBC are related to their membrane and cytoskeleton, which consists of spectrin network. Optical tweezers is able to exert very precise forces to erythrocytes using beed as a handle. In this report, we studied mechanical properties of RBC such as elasticity and viscoelasticity using single trap optical tweezers with beads with antibody coated in order to attach... 

Red blood cells are one of the most important parts of blood and their mechanical properties are so important in some diseases. Red blood cell is mostly consisted of membrane, cell skeleton, and cytoplasm which contains hemoglobin proteins. Studying these properties separately not only could give us pieces of information about cell mechanics, but also reveals a way to predict their behavior after illnesses. Optical tweezers as a tool, which can produce and measure Piconewton to Nanonewton forces, has been interesting in red blood cell parts' studying. In this thesis, at first hemoglobin is taken out gradually by using Saponin (which is enable to make some pores in the membrane) and the... 

Blood is one of the most important fluids in the human body, consisting of three parts: red blood cells, white blood cells and platelets. The mechanical properties of red blood cells are important in many diseases. The study of viscosity and other mechanical properties of red blood cells in recent years has attracted the attention of scientists in this field. The mechanical properties of red blood cells are related to the outer membrane and its cytoskeleton, and the study of these components can predict the behavior of red blood cells in diseases. The thyroid gland is located in front of the neck and secretes hormones that control many chemical processes in different parts of the body.... 

Detection of blood diseases is one of the hardest branches of medical science. The human circulatory system mainly consists of three main components namely white blood cells, red blood cells and platelets. Many diseases are diagnosed based on qualitative and quantitative evaluation of these cells, such as cell’s quantity, size, shape and the color of the cells. For example, AIDS and blood cancer cause major changes in components of immune system i.e. white blood cells. Also, some features of red blood cells change in various diseases such as iron deficiency, anemia, thalassemia and other diseases like malaria. Rapid diagnosis and treatment of these diseases has a considerable impact on the... 

Red Blood Cells (RBCs) are the main cells in human blood with a main role in mechanical properties of blood as a fluid. Several methods are developed to simulate the behavior of RBCs motion and deformation in micro-capillaries. Since in microscopic scales, using discrete models are more preferred than continuum methods, moving particle semi-implicit method (MPS) which is one of the recent innovative particle based methods, can simulate micro-fluidic flows based on Navier-Stocks equations. Although by the recent developments MPS method has turned into a considerable tool for modeling blood flow in micro-scales, but still some problems, such as commitment to use small time steps, restrict the... 

Blood is one of the most important biological organs. Red blood cell (RBC), as the most abundant cell of blood, plays an important role at life of the living bodies. Unique mechanical properties of RBCs enable them to pass through very narrow vessels. Any abnormality in these mechanical properties will disorder the function of the cell and many diseases affect these properties too. Studying the mechanical properties of RBCs is very importantin that it could result in a disease diagnostic method in a single-cell level. In this thesis we have tried to measure the viscoelastic properties of a single RBC by an optical trap through a passive method. This measurement is made through studying the... 

% of human blood makes up of plasma, that this plasma is a rich source of proteins that can be used as biomarkers for a variety of clinical diseases and laboratory research, therefore, it is necessary that the plasma be separate by a high purity percentage. Using a centrifuge It is a common method used to separate plasma from blood, but because it requires large equipment and a large volume of blood, as well as stress caused by centrifugal force, increases the probability of damage to the biomarkers, so it has been tried today that Use methods that are easy to transport and require a small amount of blood. The idea of using the microfluidic device has been put forward for the purpose of... 

In the presence of spatially non-uniform electric field, a dielectric object can experience a translational force which is known as Dielectrophoresis (DEP) force. DEP is one of the most applicable methods in sorting mechanism of different biological cells. Moreover accurately measuring the DEP force in single cell level can lead to a valuable knwoledge about the electrical properties of various cells.In this thesis, first, the other electrokinetic forces in which the Electro-Osmosis (EO) force is the important one, have to be eliminated. Regarding that this effect is strongly dependent on the frequency of external E field, we have applied the external electric field at appropriate frequency... 

In this project Deployment of multi-agent systems was studied. Maximum coverage over a point or curve or area is desired in deployment of multi-agent systems. A facility location optimization function was derived from geometrical concepts such as voronoi, n- geometric median and fermat-weber problem by optimization of which the deployment of multi-agent systems is performed. First, it is assumed that dynamic of agents is first order and second order .Next, it is assumed that agents are in a fluid. In all situations a suitable control algorithm was derived to optimize the facility location function to perform deployment. Next, another facility location function was derived by optimization...