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Flexible bactericidal graphene oxide–chitosan layers for stem cell proliferation [electronic resource]

Mazaheri, M ; Sharif University of Technology

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  1. Type of Document: Article
  2. DOI: 10.1016/j.apsusc.2014.02.099
  3. Abstract:
  4. Graphene oxide (GO)–chitosan composite layers with stacked layer structures were synthesized using chemically exfoliated GO sheets (with lateral dimensions of ∼1 μm and thickness of ∼1 nm), and applied as antibacterial and flexible nanostructured templates for stem cell proliferation. By increasing the GO content from zero to 6 wt%, the strength and elastic modulus of the layers increased ∼80% and 45%, respectively. Similar to the chitosan layer, the GO–chitosan composite layers showed significant antibacterial activity (>77% inactivation after only 3 h) against Staphylococcus aureus bacteria. Surface density of the actin cytoskeleton fibers of human mesenchymal stem cells (hMSCs) cultured on the chitosan and GO(1.5 wt%)–chitosan composite layers was found nearly the same, while it significantly decreased by increasing the GO content to 3 and 6 wt%. Our results indicated that although a high concentration of GO in the chitosan layer (here, 6 wt%) could decelerate the proliferation of the hMSCs on the flexible layer, a low concentration of GO (i.e., 1.5 wt%) not only resulted in biocompatibility but also kept the mechanical flexibility of the self-sterilized layers for high proliferation of hMSCs
  5. Keywords:
  6. Graphene ; Chitosan ; Antibacterial nanocomposites ; Stem cells
  7. Source: Journal of Applied Surface Science ; 15 May 2014, Volume 301, Pages 456–462
  8. URL: http://www.sciencedirect.com/science/article/pii/S0169433214004036