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stem-cells
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Flexible bactericidal graphene oxide–chitosan layers for stem cell proliferation [electronic resource]
, Article Journal of Applied Surface Science ; 15 May 2014, Volume 301, Pages 456–462 ; Akhavan, O ; Simchi, A. (Abdolreza) ; Sharif University of Technology
Abstract
Graphene oxide (GO)–chitosan composite layers with stacked layer structures were synthesized using chemically exfoliated GO sheets (with lateral dimensions of ∼1 μm and thickness of ∼1 nm), and applied as antibacterial and flexible nanostructured templates for stem cell proliferation. By increasing the GO content from zero to 6 wt%, the strength and elastic modulus of the layers increased ∼80% and 45%, respectively. Similar to the chitosan layer, the GO–chitosan composite layers showed significant antibacterial activity (>77% inactivation after only 3 h) against Staphylococcus aureus bacteria. Surface density of the actin cytoskeleton fibers of human mesenchymal stem cells (hMSCs) cultured...
Microfluidic systems for stem cell-based neural tissue engineering
, Article Lab on a Chip - Miniaturisation for Chemistry and Biology ; Volume 16, Issue 14 , 2016 , Pages 2551-2571 ; 14730197 (ISSN) ; Bahrami, S ; Mirshekari, H ; Moosavi Basri, S. M ; Bakhshian Nik, A ; Aref, A. R ; Akbari, M ; Hamblin, M. R ; Sharif University of Technology
Royal Society of Chemistry
2016
Abstract
Neural tissue engineering aims at developing novel approaches for the treatment of diseases of the nervous system, by providing a permissive environment for the growth and differentiation of neural cells. Three-dimensional (3D) cell culture systems provide a closer biomimetic environment, and promote better cell differentiation and improved cell function, than could be achieved by conventional two-dimensional (2D) culture systems. With the recent advances in the discovery and introduction of different types of stem cells for tissue engineering, microfluidic platforms have provided an improved microenvironment for the 3D-culture of stem cells. Microfluidic systems can provide more precise...
Controlling differentiation of stem cells for developing personalized organ-on-chip platforms
, Article Advanced Healthcare Materials ; Volume 7, Issue 2 , 2018 ; 21922640 (ISSN) ; Jafari, P ; Sheikh Hassani, M ; Heidary Araghi, B ; Mohammadi, M. H ; Ghafari, A. M ; Hassanpour Tamrin, S ; Pezeshgi Modarres, H ; Rezaei Kolahchi, A ; Ahadian, S ; Sanati Nezhad, A ; Sharif University of Technology
Wiley-VCH Verlag
2018
Abstract
Organ-on-chip (OOC) platforms have attracted attentions of pharmaceutical companies as powerful tools for screening of existing drugs and development of new drug candidates. OOCs have primarily used human cell lines or primary cells to develop biomimetic tissue models. However, the ability of human stem cells in unlimited self-renewal and differentiation into multiple lineages has made them attractive for OOCs. The microfluidic technology has enabled precise control of stem cell differentiation using soluble factors, biophysical cues, and electromagnetic signals. This study discusses different tissue- and organ-on-chip platforms (i.e., skin, brain, blood–brain barrier, bone marrow, heart,...
Synthesis of Graphene Nanosheets for Stem Cells Applications
, M.Sc. Thesis Sharif University of Technology ; Akhavan, Omid (Supervisor)
Abstract
Graphene nanogrids fabricated by graphene nanoribbons obtained through oxidative unzipping of multi-walled carbon nanotubes, used as two-dimensional selective templates for accelerated differentiation of human mesenchymal stem cells (hMSCs), isolated from umbilical cord blood, into osteogenic lineage. The biocompatible and hydrophilic graphene nanogrids showed high actin cytoskeleton proliferations that coinciding with patterns of the nanogrids. The amounts of proliferations found are slightly better than proliferation on hydrophilic graphene oxide (GO) sheets, and significantly higher than non-uniform proliferations on hydrophobic reduced graphene oxide (rGO) sheets and polydimethylsiloxane...
Agitation increases expansion of cord blood hematopoietic cells and promotes their differentiation into myeloid lineage
, Article Cytotechnology ; 2015 ; 09209069 (ISSN) ; Ebrahimi, M ; Abdekhodaie, M. J ; Sharif University of Technology
Kluwer Academic Publishers
2015
Abstract
Mechanical stress caused by agitation is one of the factors that can affect hematopoietic stem cell expansion in suspension bioreactors. Therefore, we have investigated the effects of agitation on umbilical cord blood hematopoietic stem cell (UCB-HSC) growth and differentiation. A comparison was made between various agitation rates (20, 40 and 60 rpm) in spinner-flask and cells cultured in glass petri dish as a static culture. Moreover, the fluid dynamic at various agitation rates of spinner-flask was analyzed to determine shear stress. The spinner-flask contained a rotational moving mixer with glass ball and was kept in tissue culture incubator. To reduce consumption of cytokines, UCB-serum...
Agitation increases expansion of cord blood hematopoietic cells and promotes their differentiation into myeloid lineage
, Article Cytotechnology ; Volume 68, Issue 4 , 2016 , Pages 969-978 ; 09209069 (ISSN) ; Ebrahimi, M ; Abdekhodaie, M. J ; Sharif University of Technology
Springer Netherlands
Abstract
Mechanical stress caused by agitation is one of the factors that can affect hematopoietic stem cell expansion in suspension bioreactors. Therefore, we have investigated the effects of agitation on umbilical cord blood hematopoietic stem cell (UCB-HSC) growth and differentiation. A comparison was made between various agitation rates (20, 40 and 60 rpm) in spinner-flask and cells cultured in glass petri dish as a static culture. Moreover, the fluid dynamic at various agitation rates of spinner-flask was analyzed to determine shear stress. The spinner-flask contained a rotational moving mixer with glass ball and was kept in tissue culture incubator. To reduce consumption of cytokines, UCB-serum...
Defining microRNA signatures of hair follicular stem and progenitor cells in healthy and androgenic alopecia patients
, Article Journal of Dermatological Science ; 2020 ; Nilforoushzadeh, M. A ; Youssef, K. K ; Sharifi Zarchi, A ; Moradi, S ; Khosravani, P ; Aghdami, R ; Taheri, P ; Hosseini Salekdeh, G ; Baharvand, H ; Aghdami, N ; Sharif University of Technology
Elsevier Ireland Ltd
2020
Abstract
Background: The exact pathogenic mechanism causes hair miniaturization during androgenic alopecia (AGA) has not been delineated. Recent evidence has shown a role for non-coding regulatory RNAs, such as microRNAs (miRNAs), in skin and hair disease. There is no reported information about the role of miRNAs in hair epithelial cells of AGA. Objectives: To investigate the roles of miRNAs affecting AGA in normal and patient's epithelial hair cells. Methods: Normal follicular stem and progenitor cells, as well as follicular patient's stem cells, were sorted from hair follicles, and a miRNA q-PCR profiling to compare the expression of 748 miRNA (miRs) in sorted cells were performed. Further, we...
Graphene scaffolds in progressive nanotechnology/stem cell-based tissue engineering of the nervous system
, Article Journal of Materials Chemistry B ; Volume 4, Issue 19 , 2016 , Pages 3169-3190 ; 20507518 (ISSN) ; Sharif University of Technology
Royal Society of Chemistry
2016
Abstract
Although graphene/stem cell-based tissue engineering has recently emerged and has promisingly and progressively been utilized for developing one of the most effective regenerative nanomedicines, it suffers from low differentiation efficiency, low hybridization after transplantation and lack of appropriate scaffolds required in implantations without any degrading in functionality of the cells. In fact, recent studies have demonstrated that the unique properties of graphene can successfully resolve all of these challenges. Among various stem cells, neural stem cells (NSCs) and their neural differentiation on graphene have attracted a lot of interest, because graphene-based neuronal tissue...
Stem Cell Proliferation and Differentiation in Bioreactors
, M.Sc. Thesis Sharif University of Technology ; Vosoughi, Manouchehr (Supervisor) ; Alemzadeh, Iran (Supervisor)
Abstract
In this study, differentiation of rabbit embryonic-derived mesenchymal stem cells to osteogenic cells has been characterized. Bone tissue engineering is based upon the understanding of bone tissue construct and it’s formation in-vivo, and the Preparation of tissue engineered bone constructs to repair large size defects is it’s major goal. We sought to investigate the combined effect of three elemnts of tissue engineering: cells, scaffolds and growth factors. Mesenchymal stem cells are unspecialized cells which due to their unlimited self-renewal capacity and the remarkable ability to differentiate along multiple linage pathways are natural choice for application in tissue repair and...
Accelerated differentiation of neural stem cells into neurons on ginseng-reduced graphene oxide sheets
, Article Carbon ; Volume 66 , January , 2014 , Pages 395-406 ; Ghaderi, E ; Abouei, E ; Hatamie, S ; Ghasemi, E ; Sharif University of Technology
Abstract
Asian red ginseng was used for green reduction of chemically exfoliated graphene oxide (GO) into reduced graphene oxide (rGO). The reduction level and electrical conductivity of the ginseng-rGO sheets were comparable to those of hydrazine-rGO ones. Reduction by ginseng resulted in repairing the sp 2 graphitic structure of the rGO, while hydrazine-rGO showed more defects and/or smaller aromatic domains. The ginseng-rGO sheets presented a better stability against aggregation than the hydrazine-rGO ones in an aqueous suspension. Whilst the hydrophobic hydrazine-rGO films exhibited no toxicity against human neural stem cells (hNSCs), the hydrophilic GO and ginseng-rGO films (as more...
Graphene nanogrids for selective and fast osteogenic differentiation of human mesenchymal stem cells
, Article Carbon ; Volume 59 , 2013 , Pages 200-211 ; 00086223 (ISSN) ; Ghaderi, E ; Shahsavar, M ; Sharif University of Technology
2013
Abstract
Graphene nanogrids (fabricated by graphene nanoribbons obtained through oxidative unzipping of multi-walled carbon nanotubes) were used as two-dimensional selective templates for accelerated differentiation of human mesenchymal stem cells (hMSCs), isolated from umbilical cord blood, into osteogenic lineage. The biocompatible and hydrophilic graphene nanogrids showed high actin cytoskeleton proliferations coinciding with patterns of the nanogrids. The amounts of proliferations were found slightly better than proliferation on hydrophilic graphene oxide (GO) sheets, and significantly higher than non-uniform proliferations on hydrophobic reduced graphene oxide (rGO) sheets and...
Microcarriers Development for Stem Cells Cultivation
, M.Sc. Thesis Sharif University of Technology ; Mashayekhan, Shohre (Supervisor) ; Yaghmaei, Soheyla (Supervisor)
Abstract
In general, repair and regeneration of damaged tissues or lost organ is base definition of a technology named tissue engineering. Tissue engineering technology provides innovative path to achieve better medical treatment, including different methods which manipulate natural or synthetic biomaterials mimicking stem cells environmental condition inside the body, in order to facilitate desired cell expansion and differentiation. Recently, application of cell carriers in form of microcarriers which are injectable, biodegradable and biocompatible have attracted significant attention. Due to increasing effectiveness in animal cell culture.
The aim of this work was fabrication of...
The aim of this work was fabrication of...
Cell-imprinted substrates act as an artificial niche for skin regeneration
, Article ACS Applied Materials and Interfaces ; Vol. 6, Issue. 15 , 2014 , Pages 13280-13292 ; ISSN: 19448244 ; Bonakdar, S ; Taghinejad, H ; Satarifard, V ; Heidari, M ; Majidi, M ; Sharifi, S ; Peirovi, A ; Saffar, S ; Taghinejad, M ; Abdolahad, M ; Mohajerzadeh, S ; Shokrgozar, M. A ; Rezayat, S. M ; Ejtehadi M. R ; Dalby, M. J ; Mahmoudi, M ; Sharif University of Technology
Abstract
Bioinspired materials can mimic the stem cell environment and modulate stem cell differentiation and proliferation. In this study, biomimetic micro/nanoenvironments were fabricated by cell-imprinted substrates based on mature human keratinocyte morphological templates. The data obtained from atomic force microscopy and field emission scanning electron microscopy revealed that the keratinocyte-cell-imprinted poly(dimethylsiloxane) casting procedure could imitate the surface morphology of the plasma membrane, ranging from the nanoscale to the macroscale, which may provide the required topographical cell fingerprints to induce differentiation. Gene expression levels of the genes analyzed...
An injectable platelet lysate-hyaluronic acid hydrogel supports cellular activities and induces chondrogenesis of encapsulated mesenchymal stem cells
, Article Acta Biomaterialia ; 2018 ; 17427061 (ISSN) ; Abdekhodaie, M. J ; Alvi, M ; Mousavi, A ; Karperien, M ; Dijkstra, P. J ; Sharif University of Technology
Acta Materialia Inc
2018
Abstract
Developing scaffolds that can provide cells and biological cues simultaneously in the defect site is of interest in tissue engineering field. In this study, platelet lysate (PL) as an autologous and inexpensive source of growth factors was incorporated into a cell-laden injectable hyaluronic acid-tyramine (HA-TA) hydrogel. Subsequently, the effect of platelet lysate on cell attachment, viability and differentiation of human mesenchymal stem cell (hMSCs) toward chondrocytes was investigated. HA-TA conjugates having a degree of substitution of 20 TA moieties per 100 disaccharide units were prepared and crosslinked in the presence of horseradish peroxidase and low concentrations of hydrogen...
Evaluation of differentiation quality of several differentiation inducers of bone marrow-derived mesenchymal stem cells to nerve cells by as-sessing expression of beta-tubulin 3 marker: A systematic review
, Article Current Stem Cell Research and Therapy ; Volume 16, Issue 8 , 2021 , Pages 994-1004 ; 1574888X (ISSN) ; Zahedi, F ; Hashemi, Z. S ; Khalili, S ; Sharif University of Technology
Bentham Science Publishers
2021
Abstract
Neurological diseases have different etiological causes. Contemporary, developing an ef-fective treatment for these diseases is an ongoing challenge. Cell therapy is recognized as one of the promising solutions for the treatment of these diseases. Amongst various types of stem cells, bone marrow-derived mesenchymal stem cells (BM-MSC) are known to be the most widely used stem cells. These cells are endowed with appealing properties such as the ability to differentiate into other cell types, including the muscle, liver, glial, and nerve cells. In this review study, we have systematically evaluated the ability of a variety of chemical compounds used in the last ten years to differentiate...
Electrically conductive carbon-based (bio)-nanomaterials for cardiac tissue engineering
, Article Bioengineering and Translational Medicine ; 2022 ; 23806761 (ISSN) ; Rabiee, M ; Baheiraei, N ; Ghahremanzadeh, R ; Salarian, R ; Rabiee, N ; Akhavan, O ; Zarrintaj, P ; Hejna, A ; Saeb, M. R ; Zarrabi, A ; Sharifi, E ; Yousefiasl, S ; Zare, E. N ; Sharif University of Technology
John Wiley and Sons Inc
2022
Abstract
A proper self-regenerating capability is lacking in human cardiac tissue which along with the alarming rate of deaths associated with cardiovascular disorders makes tissue engineering critical. Novel approaches are now being investigated in order to speedily overcome the challenges in this path. Tissue engineering has been revolutionized by the advent of nanomaterials, and later by the application of carbon-based nanomaterials because of their exceptional variable functionality, conductivity, and mechanical properties. Electrically conductive biomaterials used as cell bearers provide the tissue with an appropriate microenvironment for the specific seeded cells as substrates for the sake of...
Emerging bioengineering strategies for regulating stem cell fate: Scaffold physical and biochemical cues
, Article Tissue Engineering: Current Status and Challenges ; 2022 , Pages 125-156 ; 9780128240649 (ISBN) ; Mashayekhan, S ; Sharif University of Technology
Elsevier
2022
Abstract
Stem cell therapy has been introduced as an emerging approach for injured tissue regeneration. This chapter addresses developing regenerative medicine techniques for controlling stem cell behavior. Recent studies have been reviewed and novel approaches have been divided into four main categories: 3D bioprinting, lithography, microfluidics, and electrospinning. Moreover, the impact of applied biophysical and/or biochemical cues to the designed scaffold on controlling stem cell activity has been discussed. The potential of using stem cells for various soft and hard tissue regenerations has been explored in different bioengineered scaffolds and the applied techniques for controlling stem cell...
Microfluidic-based droplets for advanced regenerative medicine: current challenges and future trends
, Article Biosensors ; Volume 12, Issue 1 , 2022 ; 20796374 (ISSN) ; Heirani Tabasi, A ; Ghorbani, S ; Eyni, H ; Razavi Bazaz, S ; Khayati, M ; Gheidari, F ; Moradpour, K ; Kehtari, M ; Ahmadi Tafti, S.M ; Ahmadi Tafti, S. H ; Warkiani, M. E ; Sharif University of Technology
MDPI
2022
Abstract
Microfluidics is a promising approach for the facile and large-scale fabrication of monodispersed droplets for various applications in biomedicine. This technology has demonstrated great potential to address the limitations of regenerative medicine. Microfluidics provides safe, accurate, reliable, and cost-effective methods for encapsulating different stem cells, gametes, biomaterials, biomolecules, reagents, genes, and nanoparticles inside picoliter-sized droplets or droplet-derived microgels for different applications. Moreover, microenvironments made using such droplets can mimic niches of stem cells for cell therapy purposes, simulate native extracellular matrix (ECM) for tissue...
The use of graphene in the self-organized differentiation of human neural stem cells into neurons under pulsed laser stimulation
, Article Journal of Materials Chemistry B ; Vol. 2, Issue. 34 , 2014 , Pages 5602-5611 ; ISSN: 20507518 ; Ghaderi, E ; Sharif University of Technology
Abstract
An effective and self-organized differentiation of human neural stem cells (hNSCs) into neurons was developed by the pulsed laser stimulation of the cells on graphene films (prepared by drop-casting a GO suspension onto quartz substrates). The effects of graphene oxide (GO) and hydrazine-reduced graphene oxide (rGO) sheets on the proliferation of hNSCs were examined. The higher proliferation of the cells on the GO was assigned to its better hydrophilicity. On the other hand, the rGO sheets, which have significantly better electrical conductivity than GO, exhibited more differentiation of the cells into neurons. The pulsed laser stimulation not only resulted in an accelerated differentiation...
DNA and RNA extractions from eukaryotic and prokaryotic cells by graphene nanoplatelets
, Article RSC Advances ; Vol. 4, issue. 105 , 2014 , p. 60720-60728 ; Akhavan, O ; Shamsara, M ; Valimehr, S ; Rahighi, R ; Sharif University of Technology
Abstract
Graphene nanoplatelets with lateral dimensions of ∼50-200 nm and thicknesses <2 nm were utilized for the extraction of nucleic acids (NAs) from eukaryotic and prokaryotic cells. The graphene nanoplatelets (both chemically exfoliated graphene oxide nanoplatelets and hydrazine-reduced graphene oxide nanoplatelets) successfully extracted plasmid DNA (pDNA) from Escherichia coli bacteria, comparable to a conventional phenol-chloroform (PC) method. Furthermore, it was found that the yield of graphene nanoplatelets in genomic DNA (gDNA) and RNA extractions from embryonic stem cells (ESCs) was also comparable to the yield of the conventional methods. The effects of the graphene nanoplatelets on...