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SPR-based assay kit for rapid determination of Pb2+

Amirjani, A ; Sharif University of Technology | 2022

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  1. Type of Document: Article
  2. DOI: 10.1016/j.aca.2022.340030
  3. Publisher: Elsevier B.V , 2022
  4. Abstract:
  5. A recyclable optical nanosensor was developed by immobilizing L-tyrosine functionalized silver nanoparticles (AgNPs) on the polyethylene terephthalate (PET) substrate for rapid determination of Pb2+ ions. At first, the L-tyrosine functionalized AgNPs were assessed in the solution phase; the response time was lower than 15 s, and a limit of detection lower than 9 nM was obtained in the dynamic range of 1–1000 nM. For fabrication of the optical assay kit, the design of experiment (DOE) was used to optimize the immobilization efficiency of the nanoparticles on PET films by studying AgNO3 concentration and pH as two crucial parameters. The assay kit in optimal conditions showed a sharp localized surface plasmon resonance band suitable for sensitive determination of Pb2+. The fabricated sensor showed promising results for rapid determination of lead ions with the limit of detection value as low as 1 nM (S/N = 3). The sensor reproduced the obtained results even after three consecutive runs, which proved the recyclability of the optical assay kit. The recoveries of the spiked concentration in real samples were in the range of 95%–103%, which confirmed the applicability of the sensor in practical applications. © 2022 Elsevier B.V
  6. Keywords:
  7. Amino acid ; Heavy metal ; Lead ion ; Nanosensor ; Plasmonic ; Chemical detection ; Design of experiments ; Heavy metals ; Ions ; Metal nanoparticles ; Nanosensors ; Optical sensors ; Plastic bottles ; Silver compounds ; Surface plasmon resonance ; Amino-acids ; Functionalized ; L-tyrosine ; Lead ions ; Optical assays ; Optical nanosensors ; Plasmonics ; Recyclables ; Solution phase ; Silver nanoparticles ; Lead ; Polyethylene terephthalate ; Silver nanoparticle ; Ion ; Metal nanoparticle ; Concentration (parameter) ; Controlled study ; Experimental design ; Immobilization ; Limit of detection ; Localized surface plasmon resonance ; Nanofabrication ; Photon correlation spectroscopy ; Transmission electron microscopy ; Ultraviolet visible spectroscopy ; X ray diffraction ; X ray photoemission spectroscopy ; Procedures ; Silver ; Tyrosine
  8. Source: Analytica Chimica Acta ; Volume 1220 , 2022 ; 00032670 (ISSN)
  9. URL: https://www.sciencedirect.com/science/article/abs/pii/S0003267022006018