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Effective surface modification of MnFe2O4@SiO2@PMIDA magnetic nanoparticles for rapid and high-density antibody immobilization

Rashid, Z ; Sharif University of Technology

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  1. Type of Document: Article
  2. DOI: 10.1016/j.apsusc.2017.07.246
  3. Abstract:
  4. The present study is aimed at the synthesis of MnFe2O4@SiO2@PMIDA in terms of highly efficient sensing platform for anti-prostate specific membrane antigen (PSMA) immobilization. Superparamagnetic manganese ferrite nanoparticles were synthesized following co-precipitation method and then SiO2 shell was coated on the magnetic core with tetraethyl orthosilicate (TEOS) through a silanization reaction to prevent oxidation, agglomeration and, increase the density of OH groups on the surface of MnFe2O4. Subsequently, MnFe2O4@SiO2@PMIDA obtained as a result of the reaction between N-(phosphonomethyl)iminodiacetic acid (PMIDA) and MnFe2O4@SiO2. The reactive carboxyl groups on the surface of magnetic nanoparticles can efficiently conjugate to a monoclonal antibody, specific to PSMA, which was confirmed by enzyme-linked immune sorbent assay (ELISA). Thus, this kind of functionalized magnetic nanoparticles is promising to be utilized in the improvement of ELISA-based biosensors and also will be effective in a variety of biomedical applications such as cell separation, diagnosis, and monitoring of human diseases. © 2017 Elsevier B.V
  5. Keywords:
  6. Biosensor ; Immunoassay ; Magnetic nanoparticles ; Amino acids ; Antibodies ; Antigens ; Bioassay ; Biosensors ; Diagnosis ; Magnetism ; Medical applications ; Monoclonal antibodies ; Nanoparticles ; Precipitation (chemical) ; Silica ; Surface treatment ; Synthesis (chemical) ; Antibody immobilization ; Biomedical applications ; Immunoassay ; Magnetic nano-particles ; Manganese ferrite nanoparticles ; PMIDA linker ; Prostate-specific membrane antigens ; Tetraethyl orthosilicates ; Nanomagnetics
  7. Source: Applied Surface Science ; Volume 426 , 2017 , Pages 1023-1029 ; 01694332 (ISSN)
  8. URL: https://www.sciencedirect.com/science/article/pii/S0169433217322468