Sharif Digital Repository

Calcineurin is an essential calcium-activated serine/threonine phosphatase. The six NMR-observable methionine methyl groups in the catalytic domain of human calcineurin Aα (CNA) were assigned and used as reporters of the presence of potential cis-trans isomers in solution. Proline 84 is found in the cis conformation in most calcineurin X-ray structures, and proline 309, which is part of a highly conserved motif in phosphoprotein phosphatases, was modeled with a cis peptide bond in one of the two molecules present in the asymmetric unit of CNA. We mutated each of the two prolines to alanine to force the trans conformation. Solution NMR shows that the P84A CNA mutant exists in two forms,... 

This paper presents a state of the art design for the blade tip injection. The design includes the means, to inject high-pressure gas jet directly into a circumferential casing groove formed in the shroud adjacent to the blade tip. The casing groove is positioned over the blade tip and exceeds 30% of the blade axial chord beyond the impeller to both upstream and downstream directions. In order to validate the multi block model used in the tip gap region, main flow characteristics are verified with the experimental data for smooth casing with a design clearance of 0.5% span. Three arbitrary mass flow rates (1.75%, 2.45%, and 4.35% of choked mass flow) have been studied. The results indicate... 

The casing treatment and flow injection upstream of the rotor tip are two effective approaches in suppressing instabilities or recovering from a fully developed stall. This paper presents numerical simulations for a high-speed transonic compressor rotor, NASA Rotor 37, applying a state-of-the-art design for the blade tip injection. This is characterized by introducing a jet flow directly into the casing treatment machined into the shroud. The casing treatment is positioned over the blade tip region and exceeds the impeller axially by ∼30 per cent of the tip chord both in the upstream and in the downstream directions. To numerically solve the governing equations, the three-dimensional finite... 

Improvements in sealing mechanism between the rotating and the stationary parts of a rurbomachine can extensively reduce the endwall leakage flow. In this regard, abradable seals are incorporated into compressor and turbine blade-tip region. In a gas turbine, equipped with abradable seals, tip of the rotor blade is designed to cut into the material coating of the casing and to form a close fitted circumferential groove for the movement of the blade tip. As a result, the resistance to the leakage flow in the tip gap region increases due to smaller tip clearances (available without any rub-induced damages). Minimizing the tip clearance size can lead to an increase in performance and stability.... 

Coupling of side chain dynamics over long distances is an important component of allostery. Methionine side chains show the largest intrinsic flexibility among methyl-containing residues but the actual degree of conformational averaging depends on the proximity and mobility of neighboring residues. The 13C NMR chemical shifts of the methyl groups of methionine residues located at long distances in the same protein show a similar scaling with respect to the values predicted from the static X-ray structure by quantum methods. This results in a good linear correlation between calculated and observed chemical shifts. The slope is protein dependent and ranges from zero for the highly flexible... 

Coupling of side chain dynamics over long distances is an important component of allostery. Methionine side chains show the largest intrinsic flexibility among methyl-containing residues but the actual degree of conformational averaging depends on the proximity and mobility of neighboring residues. The 13C NMR chemical shifts of the methyl groups of methionine residues located at long distances in the same protein show a similar scaling with respect to the values predicted from the static X-ray structure by quantum methods. This results in a good linear correlation between calculated and observed chemical shifts. The slope is protein dependent and ranges from zero for the highly flexible... 

The ride comfort of high-speed trains passing over railway bridges is studied in this paper. The effects of some nonlinear parameters in a carriage-track-bridge system are investigated such as the load-stiffening characteristics of the rail-pad and the ballast, rubber elements in the primary and secondary suspension systems. The influence of the track irregularity and train speed on two comfort indicators, namely Sperling's comfort index and the maximum acceleration level, are also studied. Timoshenko beam theory is used for modelling the rail and bridge and two layers of parallel damped springs in conjunction with a layer of mass are used to model the rail-pads, sleepers and ballast. A... 

The vibration response of a Timoshenko beam supported by a viscoelastic foundation with randomly distributed parameters along the beam length and jected to a harmonic moving load, is studied. By means of the first-order two-dimensional regular perturbation method and employing appropriate Green's functions, the dynamic response of the beam consisting of the mean and variance of the deflection and of the bending moment are obtained analytically in integral forms. Results of a field measurement for a test track are utilized to model the uncertainty of the foundation parameters. A frequency analysis is carried out and the effect of the load speed on the response is studied. It is found that the... 

The response of infinite beams supported by nonlinear viscoelastic foundations subjected to harmonic moving loads is studied. A straightforward solution technique applicable in the frequency domain is presented in this paper. The governing equations are solved using a perturbation method in conjunction with complex Fourier transformation. A closed-formed solution is presented in an integral form based on the presented Green's function and the theorem of residues is used for the calculation of integrals. The solution is directed to compute the deflection and bending moment distribution along the length of the beam. A parametric study is carried out and influences of the load speed and... 

Quasi-ultrafine (quasi-UF) particulate matter (PM0.25) and its components were measured in indoor and outdoor environments at four retirement communities in Los Angeles Basin, California, as part of the Cardiovascular Health and Air Pollution Study (CHAPS). The present paper focuses on the characterization of the sources, organic constituents and indoor and outdoor relationships of quasi-UF PM. The average indoor/outdoor ratios of most of the measured polycyclic aromatic hydrocarbons (PAHs), hopanes, and steranes were close to or slightly lower than 1, and the corresponding indoor-outdoor correlation coefficients (R) were always positive and, for the most part, moderately strong (median R... 

Two carbon composite materials were prepared by mixing avocado biochar and methyl polysiloxane (MK). Firstly, MK was dissolved in ethanol, and then the biochar was added at different times. In sample 1 (R1), the time of adding biochar was immediately after dissolving MK in ethanol, and in sample 2 (R2), after 48 h of MK dissolved in ethanol. The samples were characterized by nitrogen adsorption/desorption measurements obtaining specific surface areas (SBET) of 115 m2 g−1 (R1) and 580 m2 g−1 (R2). The adsorbents were further characterized using scanning electron microscopy, FTIR and Raman spectroscopy, adsorption of vapors of n-heptane and water, thermal analysis, Bohem titration, pHpzc, and... 

This paper presents a decentralized load sharing and power management method for an islanded microgrid composed of PV units, battery units and hybrid PV/battery units. The proposed method performs all the necessary tasks such as load sharing among the units, battery charging and discharging and PV power curtailment with no need to any communication among the units. The proposed method is validated experimentally  

In quantum computing, calculations are achieved using quantum mechanics. Typically, two main phenomena of quantum mechanics (i.e., superposition and entanglement) allow quantum computing to solve some problems more efficiently than classical algorithms. The most well-known advantage of quantum computing is the speedup of some of the calculations, which have been performed before by classical applications. Scientists and engineers are attempting to use quantum computing in different fields of science, e.g., drug discovery, chemistry, computer science, etc. However, there are few attempts to use quantum computing in power and energy applications. This paper tries to highlight this gap by... 

In this work, the thermodynamic behavior of aqueous solutions containing the solutes NaCl, glucose, and/or urea is investigated. These substances are vital components for living bodies and further they are main components of blood serum. Osmotic coefficients were determined by cryoscopic measurements in single-solute and multi-solute aqueous solutions containing salts (NaCl, KCl, CaCl2), glucose, and/or urea. The results show that NaCl determines the osmotic coefficients in the urea/glucose/NaCl/water system. Investigation of the effect of different salts on osmotic coefficients revealed ion-specific effects. At physiologically important solute concentrations in typical blood serum... 

We present high-precision photometry of two transit events of the extrasolar planetary system WASP-5, obtained with the Danish 1.54-m telescope at European Southern Obseratory La Silla. In order to minimize both random and flat-fielding errors, we defocused the telescope so its point spread function approximated an annulus of diameter 40 pixel (16 arcsec). Data reduction was undertaken using standard aperture photometry plus an algorithm for optimally combining the ensemble of comparison stars. The resulting light curves have point-to-point scatters of 0.50 mmag for the first transit and 0.59 mmag for the second. We construct detailed signal-to-noise ratio calculations for defocused... 

The effect on the thermal parameters of superconducting transition-edge bolometers produced on a single crystalline SrTiO3 (STO) substrate with and without a CeO2 buffer layer was investigated. Metal-organic deposition was used to deposit the 20-nm CeO2 buffer layer, whereas RF magnetron sputtering was applied to fabricate 150-nm-thick superconducting YBa2Cu3O7-δ (YBCO) thin film. The critical transition temperature for both of the YBCO films was 90 K, and the transition width was ∼1.9 K. The bolometers fabricated from these samples were characterized with respect to the voltage phase and amplitude responses, and the results were compared with that of simulations conducted by applying a... 

Purpose: This study aimed to investigate the biomechanical impact of laminectomy on cervical intersegmental motion and load sharing using a parametric patient-specific finite element (FE) model towards providing clinicians with a viable quantitative tool for informed decision-making and improved surgical planning. Methods: Ten subject-specific nonlinear osteo-ligamentous cervical spine (C3–C7) FE models were developed using X-ray image-based algorithms. The models were used to evaluate the effect of laminectomy on lower cervical spine biomechanics for two-level (C3–C4) and three-level (C3–C5) laminectomy procedures. Results: The average cervical spine ranges of motion (ROM) for the pre-op... 

Purpose: This study aimed to investigate the biomechanical impact of laminectomy on cervical intersegmental motion and load sharing using a parametric patient-specific finite element (FE) model towards providing clinicians with a viable quantitative tool for informed decision-making and improved surgical planning. Methods: Ten subject-specific nonlinear osteo-ligamentous cervical spine (C3–C7) FE models were developed using X-ray image-based algorithms. The models were used to evaluate the effect of laminectomy on lower cervical spine biomechanics for two-level (C3–C4) and three-level (C3–C5) laminectomy procedures. Results: The average cervical spine ranges of motion (ROM) for the pre-op... 

The mean spherical approximation (MSA) model, coupled with two hard sphere models, was used to predict the activity coefficients of mixtures of electrolyte solutions at different temperatures and concentrations. The models, namely the Ghotbi-Vera-MSA (GV-MSA) and Mansoori et al.-MSA (BMCSL-MSA), were directly used without introducing any new adjustable parameters for mixing of electrolyte solutions. In the correlation step, the anion diameters were considered to be constant, whereas the cation diameters were considered to be concentration dependent. The adjustable parameters were determined by fitting the models to the experimental mean ionic activity coefficients for single aqueous... 

Traffic safety policies are still conventionally ranked on financial and environmental criteria in developing countries, yet the equity concept can be advantageously used as an integral part of the process of traffic safety policy making. Equity in transportation is defined as how appropriately and equally the impacts of transportation are distributed among different types of users. The main contribution of this research is to introduce traffic safety equity in transportation policy making. The authors advocate for a modeling framework that considers traffic safety equity as a focal point. The analytical network process overcomes the drawbacks of data intensive models that are so difficult...