Loading...
Search for:
microfluidic-system
0.015 seconds
Total 84 records
Droplet-based microfluidics in biomedical applications
, Article Biofabrication ; Volume 14, Issue 2 , 2022 ; 17585082 (ISSN) ; Besanjideh, M ; Nasiri, R ; Shamloo, A ; Nasrollahi, F ; De Barros, N. R ; Davoodi, E ; Erdem, A ; Mahmoodi, M ; Hosseini, V ; Montazerian, H ; Jahangiry, J ; Darabi, M.A ; Haghniaz, R ; Dokmeci, M.R ; Annabi, N ; Ahadian, S ; Khademhosseini, A ; Sharif University of Technology
IOP Publishing Ltd
2022
Abstract
Droplet-based microfluidic systems have been employed to manipulate discrete fluid volumes with immiscible phases. Creating the fluid droplets at microscale has led to a paradigm shift in mixing, sorting, encapsulation, sensing, and designing high throughput devices for biomedical applications. Droplet microfluidics has opened many opportunities in microparticle synthesis, molecular detection, diagnostics, drug delivery, and cell biology. In the present review, we first introduce standard methods for droplet generation (i.e. passive and active methods) and discuss the latest examples of emulsification and particle synthesis approaches enabled by microfluidic platforms. Then, the applications...
Microfluidic devices with gold thin film channels for chemical and biomedical applications: a review
, Article Biomedical Microdevices ; Volume 21, Issue 4 , 2019 ; 13872176 (ISSN) ; Rabiee, N ; Rabiee, M ; Bagherzadeh, M ; Tahriri, M ; Tayebi, L ; Hamblin, M. R ; Sharif University of Technology
Springer New York LLC
2019
Abstract
Microfluidic systems (MFS) provide a range of advantages in biomedical applications, including improved controllability of material characteristics and lower consumption of reagents, energy, time and money. Fabrication of MFS employs various materials, such as glass, silicon, ceramics, paper, and metals such as gold, copper, aluminum, chromium and titanium. In this review, gold thin film microfluidic channels (GTFMFC) are discussed with reference to fabrication methods and their diverse use in chemical and biomedical applications. The advantages of gold thin films (GTF) include flexibility, ease of manufacture, adhesion to polymer surfaces, chemical stability, good electrical conductivity,...
Numerical simulation of a microfluidic system for regular glucose measurement
, Article 26th National and 4th International Iranian Conference on Biomedical Engineering, ICBME 2019, 27 November 2019 through 28 November 2019 ; 2019 , Pages 60-65 ; 9781728156637 (ISBN) ; Saidi, M. S ; Shahrokhian, S ; Sharif University of Technology
Institute of Electrical and Electronics Engineers Inc
2019
Abstract
The aim of this research is to design an implantable integrated microfluidic system in order to regularly measure the glucose level in the human body, nonenzymatically, using the microdialysis method. The main compartments of this system are a micropump, array of hollow microneedles and an electrochemical sensor. At the base of the microneedles, there are located semipermeable membranes, that when the pumped dialysis fluid passes over them, the glucose of the interstitial fluid diffuses into the dialysis fluid and then, in the sensor section, it is measured nonenzymatically using the amperometry method. Both the arrangement of the miconeedles and the amount of the dialysis fluid flow are...
The effects of thymus plant extracts on single breast cancer cell morphology in the microfluidic channel
, Article 2018 IEEE EMBS Conference on Biomedical Engineering and Sciences, IECBES 2018, 3 December 2018 through 6 December 2018 ; 2019 , Pages 647-651 ; 9781538624715 (ISBN) ; Mansor, M. A ; Alsadat Rad, M ; Soo-Beng Khoo, A ; Ahmad, M ; Marzuki, M ; Physiological Measurement; Sarawak Convention Bureau ; Sharif University of Technology
Institute of Electrical and Electronics Engineers Inc
2019
Abstract
Microfluidics based systems could be useful for drug discovery as they allow for miniaturization and could potentially be run as multiple parallel cell based assays. Such miniaturization allows assays at single cell level and reduces the amount of test material needed, which, in the case of natural product extracts, simplifies the preparation. Thyme species extracts have been reported to show some promising anti-cancer effects. In the present work, we used a microfluidics based system to study the effects of Thymus kotschyanusm Boiss plant extract on two human breast cancer cells lines which are MDA-MB-231 and MCF-7. For better understanding a single cancer cell death mechanism and a flow...
Microfluidic Investigation of Hydrogen-Water Flow at Pore-Scale for Subsurface Hydrogen Storage
, M.Sc. Thesis Sharif University of Technology ; Mahani, Hassan (Supervisor) ; Ayatollahi, Shahaboddin (Supervisor) ; Zivar, Davood (Co-Supervisor)
Abstract
Hydrogen storage in porous subsurface formations, such as aquifers or depleted hydrocarbon reservoirs because of their high storage capacity, has gained momentum as a promising approach to balance the renewable energy supply and demand. However, the poor understanding of hydrogen flow dynamics in porous media is the main obstacle to the development and widespread application of underground hydrogen storage (UHS). For example, the main uncertainty is lack of detailed understanding of hydrogen flow dynamics in the natural porous media which leads to the unknown volume of recoverable hydrogen for this cyclic process. In this research, by developing a visual microfluidic apparatus to handle...
Design, Simulation, and Fabrication of DEP-Based Microfluidic Chip for Bioparticles Separation
, M.Sc. Thesis Sharif University of Technology ; Fardmanesh, Mehdi (Supervisor)
Abstract
Microfluidics, employing fluids in structures with micrometer dimensions, is an emerging technology that has brought great opportunities to develop various devices including lab-on-a-chip systems. Lab-on-a-chip systems have the advantages of integrating several stages of different analytical methods, application in diverse fields, consumption of small volumes of samples, and portability. One of the crucial applications of lab-on-a-chip devices is sorting cells and biological particles, done by different methods. In separator devices, high separation efficiency and repeatability are critical. When a neutral particle is polarized in a non-uniform electric field, the dielectrophoresis force...
Investigating the Effect of Electric Fields on the Efficiency of Phase Micro Separator Using Simulation
, M.Sc. Thesis Sharif University of Technology ; Mohammadi, Ali Asghar (Supervisor)
Abstract
In this study, the performance of electrocoalescence in a microfluidic device was investigated through numerical simulations. The microfluidic device consisted of two immiscible fluids, a continuous phase, and a dispersed phase, which were modeled as incompressible Newtonian fluids. The interface between the two phases was tracked using a phase-field model. A flow-focusing geometry was employed to crate water droplets in oil. The dispersed droplets were then pass over a set of electrodes. The merging of two adjacent droplets in the microfluidic device was observed by applying a constant electric field. The voltage of 60 V is the lowest voltage at which the regular merging of pairs of drops...
Micromodel Investigation of the Non-Monotonic Effect of Injection Water Salinity on Wettability and Oil Recovery
, M.Sc. Thesis Sharif University of Technology ; Mahani, Hassan (Supervisor)
Abstract
Based on numerous laboratory and field evidence, low-salinity waterflooding or engineered salinity waterflooding can lead to enhanced oil production. According to the literature, the mechanisms involved in this process can be divided into two general categories: fluid-fluid interactions and solid-liquid interactions. These mechanisms are caused by intermolecular and electrostatic forces at the rock and fluid interfaces. The most important controlling factor of the electrostatic forces is the concentration of ions at/near fluids-rock interface. Therefore, improving the concentration of ions causes a shift in wettability toward a more water-wetting state, eventually leading to increased oil...
Numerical Study of Enhancement of Inflection Point Focusing for Blood Cell Separation
, M.Sc. Thesis Sharif University of Technology ; Moosavi, Ali (Supervisor) ; Sadrhosseini, Hani (Co-Supervisor)
Abstract
Today, the determination and therapy of numerous illnesses, including malignant growth, relies upon the information and assessment of platelets, so blood testing and cell examination is fundamental to survey the movement of disease. The lab on a chip innovation is utilized as an extremely productive device in cell studies. The lab on a chip is used as a foundation and a substrate for making a legitimate stream for cell processes in medication. This innovation is a gadget or framework with millimeters or centimeters aspects like a chip, and it performs research facility handling on a micron-scale. In spite of the fact that it has a few impediments, it has so many values; for example, it...
Design and Optimization of an Intelligent Impedance-based Flow Cytometer for POCT Applications
, M.Sc. Thesis Sharif University of Technology ; Fardmanesh, Mehdi (Supervisor) ; Annabestani, Mohsen (Co-Supervisor)
Abstract
Microfluidics is the science of fabricating systems interfacing fluids of small volume and size ranging from microliters (10-6) to picolitres (10-12). Due to its remarkable features, such as low sample and reagent consumption, high sensitivity, low cost, automated devices, and short experimental time, microfluidic chips have been widely used as laboratory assays. As a term often used and described in “lab on a chip” and “organ on a chip” technologies, microfluidic chips have been used in the fast and frugal diagnosis of infectious diseases such as HIV, microscale surgery on single cells and tiny organisms and cell culture.The design, simulation, optimization, and fabrication of an...
Simulating of Droplet Formation and Calculation of Interfacial Tension by Microfluidic Method
, M.Sc. Thesis Sharif University of Technology ; Mohammadi, Ali Asghar (Supervisor)
Abstract
Emulsions are the intermittent dispersion of liquid droplets that have many applications such as polymerization, dyeing, cosmetics, food industry, etc. Droplets can be created in a variety of ways, but the most important point in emulsification and the greatest efficiency of its applications is when the formed droplets are uniform. In the emulsification process, there is a key parameter called interfacial tension, which affects the behavior of the formed droplets, such as their stability and morphology, and its precise measurement is very effective in the quality control of the mentioned applications. Be. In this research, droplet formation in microfluidic systems due to uniform droplet...
Design and Fabrication of a 3D Multiparticle Microfluidic System
, M.Sc. Thesis Sharif University of Technology ; Fardmanesh, Mehdi (Supervisor) ; Annabestani, Mohsen (Co-Supervisor)
Abstract
Microfluidic technology is a field of science that deals with the design, implementation, optimization, and testing of fluid systems in small dimensions. This technology has made significant progress in various fields in the last decade, especially in medical engineering. As an interdisciplinary scientific field, this growing technology has many applications in medicine, pharmaceuticals, chemistry, and electronic industries. One of the primary applications of microfluidic devices is the development of "lab-on-a-chip" systems as point-of-care diagnostic tools, such as rapid diagnosis during surgery. A microfluidic system includes various functional modules: sample preparation and fluid...
Design and Development of Non-Newtonian Droplet-based Logic Microfluidics Using Passive Method
, Ph.D. Dissertation Sharif University of Technology ; Moosavi, Ali (Supervisor) ; Kazemzadeh Hannani, Siamak (Supervisor)
Abstract
Droplet-based microfluidic logic gates have many applications in diagnostic tests and biosciences due to their automation and cascading ability. Although most biological fluids, such as blood, exhibit non-Newtonian properties, all previous studies in this field have been with Newtonian fluids. Additionally, none of the previous work has studied the functional area of logic gates. In the present work, AND-OR logic gate with power-law fluid is considered. The effect of important parameters such as non-Newtonian fluid properties, droplet length, capillary number, and geometrical properties of the microfluidic system on the operating region of the system has been investigated. The results show...
3d Design of a Microfluidic Chip for Anticancer Drugs Screening
, M.Sc. Thesis Sharif University of Technology ; Mashayekhan, Shohreh (Supervisor) ; Saadatmand, Maryam (Supervisor)
Abstract
Nowadays, advanced and inexpensive pre-clinical methods for investigating the effects of anti-cancer drugs are expanding. One of the latest three-dimensional laboratory modeling for evaluating the effects of drugs is the use of tumor-on-chip technology, which actually models the physiological system of the body through three-dimensional scaffolds, multicellular cultures, and shaped vascular systems. In this study, three-dimensional culture of cancer cells was performed in the form of spheroids. A chip of U-shaped microstructures with and without gaps was used to trap cells and form cancer spheroids. We simulated the simultaneous effect of drug and oxygen concentration distribution inside the...
Synthesis of Amine-Functionalized BN Quantum Dots for Active Targeting of SKOV3 Cells and Cell Imaging in a Microfluidics System
, M.Sc. Thesis Sharif University of Technology ; Simchi, Abdolreza (Supervisor) ; Mohammadi, Mohammad Reza (Supervisor)
Abstract
The biocompatibility and chemical stability of boron nitride nanomaterials surpass those of carbon nanomaterials. In addition, boron nitride nanomaterials share the same structure as graphene materials, making them a suitable replacement for graphene in biological applications. In this study, SKOV3 cancer cells were actively targeted using amine-functionalized boron nitride quantum dots that were covalently modified by CD133 antibody. Under the condition that they are not conjugated with antibody, these nanoparticles were also incorporated into the microfluidic device designed to image MCF7 cancer cells. These nanoparticles were produced via solvothermal synthesis in an organic solvent...
CFD-Assisted Design of Microreactor and Evaluation for Continuous Drug Synthesis
, M.Sc. Thesis Sharif University of Technology ; Kazemeini, Mohammad (Supervisor) ; Hosseinpour, Vahid (Co-Supervisor)
Abstract
Manufacturing in many industries, such as electronics, petrochemical, automotive, and food, has long relied on continuous approaches, while the pharmaceutical industry has traditionally been processed in batch reactors. Since the beginning of the third millennium, continuous drug synthesis has received attention due to its multiple advantages over batch systems, which include process safety, adaptability, controllability, economic efficiency, scale-up, and enhancing performance. In this project, the pharmaceutical intermediate 1,4-benzodiazepine, which is usable as a precursor of diazepam and flurazepam, is continuously synthesized in a 400 microliters integrated micromixer and microreactor...
Improving the Accuracy of a Microparticle Biosensor by Artificial Intelligence
, M.Sc. Thesis Sharif University of Technology ; Taghipoor, Mojtaba (Supervisor)
Abstract
In this research, the ability of artificial intelligence to improve the biosensor performance of a microfluidic system has been investigated. Coulter counter is a microfluidic system that measures the concentration of particles in a fluid using signals obtained from a biosensor. The method of this system is called " Resistive Pulse Sensing (RPS)" method. The pulses in the Coulter counter signals are affected by the number, shape, size and speed of particles passing through the orifice. The pulse of two particles of the same size and different shape in these systems are very similar and this makes it difficult for an operator to distinguish the type of particle. Another disadvantage of...
Investigation of 3D Printed Microfluidic System for Continues Drug Synthesis
, M.Sc. Thesis Sharif University of Technology ; Kazemeini, Mohammad (Supervisor) ; Hosseinpour, Vahid (Co-Supervisor)
Abstract
In recent years, batch systems were often used in the synthesis of pharmaceuticals. Nowadays, due to the possibility of more optimal conditions in flow systems, such as better mixing and optimal performance of heat transfer and mass transfer, process safety, reduction of retention time, and ease in increasing the scale of the process, the use of flow systems has been expanded. B-hydroxysulfone is a pharmaceutical intermediate in the production of bicalutamide, which is used in the treatment of prostate cancer and is used in many organic and biological processes. The reaction time in batch systems is about 17 to 20 hours, and in the flow system, the retention time has reached about 180-90...
Fabrication of a Centrifugal Microfluidic System for Cell-free DNA Isolation from whole Blood as a Biomarker
, M.Sc. Thesis Sharif University of Technology ; Saadatmand, Maryam (Supervisor) ; Garshasbi, Masoud (Co-Supervisor)
Abstract
Today, medical science has come to the conclusion that the best time to start treating a disease is in its early stages. Therefore, the topic of early detection has been widely welcomed in scientific circles. Today, one of the safest methods for early detection in medicine is the study of biomarkers. These biomarkers include CTC, cfRNA, cfDNA, Exosome, etc. For instance, in the discussion of early cancer detection, the US Food and Drug Administration has so far identified two biomarkers, cfDNA and CTC, as the safest markers. The cfDNA marker is also widely used not only in the diagnosis of cancer, but also in the field of sex diagnosis and fetal Down syndrome in the first weeks of pregnancy....
Design, Fabrication and Evaluation of Polymeric Microcapsules Containing Cells to Use in Cell Therapy in Heart
, M.Sc. Thesis Sharif University of Technology ; Mashayekhan, Shohreh (Supervisor) ; Sharifi, Ali Mohammad (Supervisor) ; Khanmohammadi, Mehdi (Co-Supervisor)
Abstract
Because of the inability of conventional methods to regenerate the infarcted part of the heart, regenerative medicine based on using scaffolds and hydrogels incorporation with cells and biological factors emerges as a promising approach. Micro-structures, especially hollow microcapsules created by the microfluidic system, has received a great deal of attention due to their inspired biomimetic structures, providing complete coverage cells, and preventing an immune response, consequently. This study aimed to use a microfluidic system to fabricate hollow microcapsules and investigate the behavior of rat cardiomyoblast cells (H9C2) along with exosomes within these structures. These structures'...