Sharif Digital Repository

Due to great commercial application of protease, it is necessary to study kinetic characterization of this enzyme in order to improve design of enzymatic reactors. In this study, mathematical modeling of protease enzyme production kinetics which is derived from Bacillus licheniformis BBRC 100053 was studied (at 37°C, pH 10 after 73 h in stationary phase, and 150 rpm). The aim of the present paper was to determine the best kinetic model and kinetic parameters for production of protease and calculating K i (inhibition constant) of different inhibitors to find the most effective one. The kinetic parameters K m (Michaelis-Menten constant) and V m (maximum rate) were calculated 0.626 mM and... 

The corrosion behavior of different grades of commercial aluminum such as AA1040, AA5083, AA6060 and AA7075 in ZnO-containing 4 M NaOH has been determined by using open circuit potential-time measurements (OCP), galvanostatic and potentiodynamic polarization and electrochemical impedance spectroscopy (EIS). The results of scanning electron microscopy (SEM) and energy dispersive analysis of X-ray (EDAX) reveal that ZnO produces the inhibition effect by the formation of a zinc-containing deposit layer on the surface of aluminum electrodes. Although the influence of zincating on the performance of aluminum alloys and considering the amount of alloying elements such as zinc, magnesium and... 

This work is concerned with the monitoring of ultrasound effects on inhibition, as well as the reversible kinetics modeling of asphaltene flocculation in toluene-n-alkane systems, which has been rarely reported in the literature. A crude oil sample was exposed to ultrasound waves, and then the colloidal structural evolutions of flocculated asphaltene particles induced by addition of n-alkane were studied, using a confocal microscopy. Observations confirmed that radiation of ultrasound can change the irreversibility of asphaltene flocculation in crude oil. To interpret the kinetics of asphaltene flock aggregation, the Smoluchowski model was used, and the time dependent size distribution of... 

This paper presents a new method for spectrophotometirc detection of sulfide applying fungal peroxidase immobilized on sodium alginate. The sensing scheme was based on decrease of the absorbance of the orange compound, purpurogallin produced from pyrogallol and H2O2 as substrates, due to the inhibition of peroxidase by sulfide. Absorbance of purpurogallin was detected at 420nm by using a spectrophotometer. The proposed method could successfully detect the sulfide in the concentration range of 0.6-7.0μM with a detection limit of 0.4μM. The kinetic parameters of Michaelis-Menten with and without sulfide were also calculated. Possible inhibition mechanism of peroxidase by sulfide was deduced... 

Amyloid beta fibrillation can lead to major disorder of neurons processes and is associated with several neuronal diseases (e.g., Alzheimer's disease). We report here an importance of slight temperature changes, in the physiological range (35-42 °C), on the amyloid fibrillation process in the presence and absence of hydrophilic (silica) and hydrophobic (polystyrene) nanoparticles (NPs). The results highlight the fact that slight increases in temperature can induce inhibitory and acceleratory effects of hydrophobic and hydrophilic NPs on the fibrillation process, respectively. Using further in vivo considerations, the outcomes of this study can be used for considerable modifications on the... 

The effects of temperature, seed mass and agitation rate on agglomeration kinetics of aluminum hydroxide in Bayer process have been studied in a batch system. Collected raw data were analyzed and the kinetics data of agglomeration were derived through simulation of the process using a pre-developed software. The results showed that agglomeration kinetics constant (agglomeration kernel) increases with increase in temperature and agitation rate. Moreover a maximum value of agglomeration rate versus added seed mass was observed. Furthermore the magnitude of calculated activation energy of agglomeration was close to that of growth  

In the present work, an amperometric inhibition biosensor for the determination of sulfide has been fabricated by immobilizing Coprinus cinereus peroxidase (CIP) on the surface of screen printed electrode (SPE). Chitosan/acrylamide was applied for immobilization of peroxidase on the working electrode. The amperometric measurement was performed at an applied potential of -150. mV versus Ag/AgCl with a scan rate of 100. mV in the presence of hydroquinone as electron mediator and 0.1. M phosphate buffer solution of pH 6.5. The variables influencing the performance of sensor including the amount of substrate, mediator concentration and electrolyte pH were optimized. The determination of sulfide... 

Interactions of chemically exfoliated graphene oxide (GO) nanosheets and Escherichia coli bacteria living in mixed-acid fermentation with an anaerobic condition were investigated for different exposure times. X-ray photoelectron spectroscopy showed that as the exposure time increased (from 0 to 48 h), the oxygen-containing functional groups of the GO decreased by ∼60%, indicating a relative chemical reduction of the sheets by interaction with the bacteria. Raman spectroscopy and current-voltage measurement confirmed the reduction of the GO exposed to the bacteria. The reduction was believed to be due to the metabolic activity of the surviving bacteria through their glycolysis process. It was... 

Based on series of crude oil rheological properties and asphaltene flocculation confocal microscopy analysis, Najafi et al., (2011) reported the existence of an optimum radiation time at which asphaltenic crude oils reach the minimum kinematic viscosity. Accordingly, they proposed the idea of asphaltene flocculation inhibition due to wave radiation.The present investigation is a continuous effort to provide more information about the process of flocculation inhibition. Confocal microscopy and rheological analyses are performed on different crude oils to prove the repeatability of the observed phenomena. The asphaltene content analysis was done based on IP143 procedure, which provides more... 

Copper corrosion in sodium dodecyl sulphate (SDS) solutions and carbon nanotube (CNT) nanofluids were studied by potentiodynamic polarization. For the corrosion current densities calculations, Koutecky-Levich equation was modified to model the combined charge and mass transport. 0.005. M SDS reduced the copper corrosion current density by 81%. Higher SDS concentrations enhanced corrosion. Stirring SDS solutions increased the corrosion current density by ∼75%. By adding CNT to SDS solution, the corrosion current density first decreased and then remained constant. Stirring CNT nanofluids didn't change the corrosion current density. An adsorbed CNT layer on copper controlled the corrosion... 

In this study the ability of Acidithiobacillus ferrooxi-dans, with regard to the biorecovery of heavy metals in shake flask has been investigated. Adaptation experiments with the single metal ions Ni, Co, V, Mo, W and a mixture of the first four metal ions in the medium was developed through serial sub-culturing. Adaptation showed that A. ferrooxidans could tolerate up to 2.3 g/l Ni, 1.4 g/l Co, 1.4 g/l V, 0.045 g/l Mo and 0.005 g/l W, singly. In the presence of multi-metals considering a mixture of Ni-Co-V-Mo, the bacteria was able to tolerate up to 1.5 g/l Ni, 0.8 g/l Co, 0.8 g/l V and 0.05 g/l Mo in steps of 50-100 mg/l for Ni, Co and V, while for Mo and W with increments in concentration... 

Polyvinyl alcohol/sodium alginate/nano silver (PVA/SA/Ag) composite films were made by solution casting method. Gamma irradiation was used to synthesize silver nanoparticles in situ via reduction of silver nitrate without using harmful chemical agents for biomedical applications. UV-vis and XRD results demonstrated that spherical silver nanoparticles were produced even at low irradiation dose of 5. kGy. By increasing irradiation dose, more nanoparticles were synthesized while no PVA hydrogel was formed up to 15. kGy. Also the size of nanoparticles was reduced with increasing gamma dose evidenced by higher release rate of silver nanoparticles in lukewarm water and SEM images. Comparing SEM...