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Design and Manufacture of a Scaffold with a Drug Delivery System for a Better Tissue Wound Healing Process
Shaygani, Hossein | 2022
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- Type of Document: M.Sc. Thesis
- Language: Farsi
- Document No: 55625 (08)
- University: Sharif University of Technology
- Department: Mechanical Engineering
- Advisor(s): Shamloo, Amir; Aryanpour, Masoud
- Abstract:
- Articular cartilage is devoid of blood vessels, lymphatics, and nerves which gives it a very limited intrinsic healing and repair capabilities. Being under constant harsh biomechanical environment, makes maintaining the health of articular cartilage a vital principle in having healthy joints. Tissue engineering as a method for regeneration of damaged tissue have attracted a lot of attention. Articular cartilage engineered scaffolds act as a macro scale drug delivery system which in addition to having a good mechanical properties similar to that of cartilage tissue, have to provide a highly porous environment for cell migration and proliferation. The aim of this study is to fabricate a drug delivery system to repair damaged tissue. To this end, an injectable hydrogel scaffold with two interpenetrating polymer networks consisting of chitosan and silk fibroin was fabricated and loaded with microspheres containing methylprednisolone acetate. To create an interpenetrating polymer network, both chitosan and silk fibroin were crosslinked with β-glycerophosphate and sodium dodecyl sulfate, respectively. Afterward, the polymer network ratios were optimized. From the optimization results, the sample with silk fibroin to chitosan polymer network ratio of 2 to 1, having Younge’s modulus of 46 kPa, 52% cell viability, 50% degradation rate and 81% drug release rate during 5 weeks was chosen as the optimal sample. To further study the effect of this scaffold on the living tissue, rabbits were randomly assigned to the optimal scaffold and control groups. After 10 weeks, the rabbits with scaffolds showed better locomotion compared to the control group. Furthermore, the diameter of the defects during this period decreased to 3.9 and 4.8 for the scaffold group and the control group, respectively
- Keywords:
- Microspheres ; Silk Fibroin ; Chitosan ; Interpenetrating Polymer Network ; Tissue Engineering ; Hydrogel Scaffold ; Cartilage Tissue Engineering ; Combined Drug Delivery System ; Methylprednisolone Acetate
- محتواي کتاب
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- چکیده
- فهرست مطالب
- فهرست جدولها
- فهرست تصویرها
- فصل1 : پیشدرآمد
- فصل2 مفاهیم پایه
- 2-1 مقدمه
- 2-2 بافت غضروف
- 2-3 روشهای درمانی کنونی جراحی مفصل
- 2-4 روشهای سنتی دارورسانی
- 2-5 روشهای نوین دارورسانی
- 2-6 مهندسی بافت
- 2-7 فرایند ژل شدن فیبروئین ابریشم با سدیم دودسیل سولفات
- 2-8 فرایند ژل شدن کیتوسان با بتاگلیسروفسفات
- 2-9 متیل پردنیزولون استات
- فصل3 پیشینه پژوهش
- فصل4 روش پژوهش
- 4-1 مقدمه
- 4-2 روش و مواد پیشنهادی
- 4-3 مراحل پژوهش
- 4-4 مواد و تجهیزات لازم
- 4-5 استخراج فیبروین ابریشم
- 4-6 ساخت داربست بدون دارو
- 4-7 روشهای بررسی داربست پیشنهادی
- 4-7-1 زمان ژل شدن
- 4-7-2 نرخ تورم
- 4-7-3 ظرفیت جذب آب
- 4-7-4 درصد کراسلینک شده
- 4-7-5 نرخ زیستتخریبپذیری
- 4-7-6 بررسی مورفولوژی داربست
- 4-7-7 طیفسنجی تبدیل فوریه مادونقرمز
- 4-7-8 آزمون مکانیکی فشار
- 4-7-9 آزمون رئومتری
- 4-7-10 نرخ رهایش دارو
- 4-7-11 آزمون آنتیباکتریال
- 4-7-12 زیستسازگاری
- 4-7-13 آزمون داربست در بافت زنده
- فصل5 بررسی و تحلیل نتایج آزمونها
- 5-1 مقدمه
- 5-2 بهینهسازی درصد بتاگلیسروفسفات
- 5-3 بهینهسازی درصد سدیم دودسیل سولفات
- 5-4 زمان ژل شدن داربست ترکیبی
- 5-5 تورم
- 5-6 جذب آب
- 5-7 درصد کراسلینک شدن
- 5-8 آزمایش زیستتخریبپذیری
- 5-9 تحلیل مورفولوژی
- 5-10 طیفسنجی تبدیل فوریه مادونقرمز
- 5-11 مکانیکی فشار
- 5-12 رئومتری
- 5-13 نرخ رهایش دارو
- 5-14 آزمون آنتیباکتریال
- 5-15 زیستسازگاری
- 5-16 آزمون داربست در بافت زنده
- فصل6 نتیجهگیری و پیشنهادها