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Investigation of Biocompatibility and Biodegradability of Porous Chitosan Scaffold in Nerve Tissue Engineering

Dabaghi, Mohammad Hossein | 2014

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 45958 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Mashayekhan, Shohreh; Ramezani Saadat, Ahmad
  7. Abstract:
  8. Nerve repair plays a very prominent and significant role among the efforts that have been made to integrate the concepts of tissue engineering in strategies to repair almost all parts of the body. This is partly due to the complexity of the nervous anatomy system and its function as well as the inefficiency of conventional repair methods that are based on a component of biomaterials or cells alone. Studies show that electrical stimulation can enhance the nerve regeneration process; so the use of conducting polymers has attracted much attention for the construction of neural tissue engineering scaffolds. In this study, the electrical properties of neurons and the effects of electrical stimulation on neurons are the subjects to be studied. In addition, polypyrrole (PPy) and polyaniline (PANI) are two conductive polymers that are widely used in nerve tissue engineering and especially in research on different applications, and their performance has been proven in this regard.This study examined the biocompatibility and biodegradability properties of conductive scaffolds made of gelatin, chitosan and polyaniline composite and graphene. Then, we examined the irritability of conductive scaffolds, under direct current and step current, and its effect on the growth of Schwann cells in the study. The results of the biodegradation assay and MTT assay show that scaffolds containing 2.5 weight percent of conductive polymer are ideal for nerve tissue engineering. Results obtained from MTT assay and SEM photos suggest that electrical stimulation, either with direct current or with step current, has a significant positive impact on the growth of Schwann cells
  9. Keywords:
  10. Biocompatibility ; Biodegradation ; Electrical Stimulation ; Schwann Cell ; Nerve Tissue Engineering

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