Multi- and Single-cellular Encapsulation within Microchannels for Effective Cell Lysis and DNA Extraction and Purification, M.Sc. Thesis Sharif University of Technology ; Shamloo, Amir (Supervisor)
Abstract
In this study a droplet-based microfluidic system is desighned and fabricated to effectiverly lyse MCF7 cells and extract and purify their DNAs. The main purpose of this study is to transfer all the steps from macro scale to a microfluidic system containing a fluidic chip. This system is a semi automatic system and every part of the lysis and purification process is performed in one step. The first step is to encapsulate single cells and multi cells inside the droplets. By controlling the concentration of the cell solution, the number of encapsulated cells inside the droplet is efficiently and easily controlled and the cells were encapsulated as single cells and as multi cells inside the...
Cataloging briefMulti- and Single-cellular Encapsulation within Microchannels for Effective Cell Lysis and DNA Extraction and Purification, M.Sc. Thesis Sharif University of Technology ; Shamloo, Amir (Supervisor)
Abstract
In this study a droplet-based microfluidic system is desighned and fabricated to effectiverly lyse MCF7 cells and extract and purify their DNAs. The main purpose of this study is to transfer all the steps from macro scale to a microfluidic system containing a fluidic chip. This system is a semi automatic system and every part of the lysis and purification process is performed in one step. The first step is to encapsulate single cells and multi cells inside the droplets. By controlling the concentration of the cell solution, the number of encapsulated cells inside the droplet is efficiently and easily controlled and the cells were encapsulated as single cells and as multi cells inside the...
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