Loading...

Design and Fabrication of Microfluidic Based Systems for Electrical Properties Measurement of Bio-Particles

Aghasizadeh, Afarin | 2019

510 Viewed
  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 52517 (05)
  4. University: Sharif University of Technology
  5. Department: Electrical Engineering
  6. Advisor(s): Fardmanesh, Mehdi
  7. Abstract:
  8. Microfluidics is a novel technology that brought a revolution in the field of chemistry and biology. This technology allows precise control and manipulation of fluids and particles in sub-millimeter bio-compatible channels. This makes microfluidics a powerful tool to study and examine bio-particles and bio-processes. One of the most prominent areas in microfluidics is the integration of developed measurement tools and devices of the electrical engineering field, with microfluidic chips. The purpose of this thesis is to measure the electrical impedance spectrum of bio-particles with high precision and in a wide bandwidth. These valuable spectrums are useful in many applications such as diagnosis and treatment of diseases, assistive reproductive technologies, neuroscience, genetics, etc. For this purpose, in the beginning, different structures and designs of a microfluidic chip with microelectrodes were simulated and analysed. In this step, an optimized design of coplanar electrodes had a capacitance of 40 fF with 0.1 fF difference in the presence of the reference particle (polystyrene bead with 4um radius). The capacitance for an optimized design of parallel electrodes was 80 fF with 6 fF variation. Assessment of this amount of variations in capacitance, requires an ultrasensitive circuit with high gain and ultra-low noise. The designed and implemented circuit for this thesis was a trans-impedance amplifier with 100 kΩ gain and 30 pA current noise. Next, three different configuration of microfluidic chips and microelectrodes were fabricated and analyzed alongside the challenges and the applied solutions for that matter. Also, a syringe pump with an injection/suction rate of 0.71 μL/min was designed and fabricated. Finally, using the fabricated system, impedance spectrum of polystyrene bead and baker’s yeast cells were measured from 10 kHz to 10 MHz
  9. Keywords:
  10. Measurement ; Biocompatibility ; Electrical Impedance ; Spectrometry ; Microfluidic System ; Microelectrode Array

 Digital Object List

 Bookmark

No TOC