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Microfluidics-Enabled multimaterial maskless stereolithographic bioprinting

Miri, A. K ; Sharif University of Technology | 2018

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  1. Type of Document: Article
  2. DOI: 10.1002/adma.201800242
  3. Publisher: Wiley-VCH Verlag , 2018
  4. Abstract:
  5. A stereolithography-based bioprinting platform for multimaterial fabrication of heterogeneous hydrogel constructs is presented. Dynamic patterning by a digital micromirror device, synchronized by a moving stage and a microfluidic device containing four on/off pneumatic valves, is used to create 3D constructs. The novel microfluidic device is capable of fast switching between different (cell-loaded) hydrogel bioinks, to achieve layer-by-layer multimaterial bioprinting. Compared to conventional stereolithography-based bioprinters, the system provides the unique advantage of multimaterial fabrication capability at high spatial resolution. To demonstrate the multimaterial capacity of this system, a variety of hydrogel constructs are generated, including those based on poly(ethylene glycol) diacrylate (PEGDA) and gelatin methacryloyl (GelMA). The biocompatibility of this system is validated by introducing cell-laden GelMA into the microfluidic device and fabricating cellularized constructs. A pattern of a PEGDA frame and three different concentrations of GelMA, loaded with vascular endothelial growth factor, are further assessed for its neovascularization potential in a rat model. The proposed system provides a robust platform for bioprinting of high-fidelity multimaterial microstructures on demand for applications in tissue engineering, regenerative medicine, and biosensing, which are otherwise not readily achievable at high speed with conventional stereolithographic biofabrication platforms. © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
  6. Keywords:
  7. Digital light prototyping ; Digital micromirror devices ; Multimaterials ; Biocompatibility ; Cell engineering ; Digital devices ; Fluidic devices ; Hydrogels ; Microfluidics ; Polyethylene glycols ; Stereolithography ; Tissue engineering ; Bioprinting ; Digital micro-mirror device ; High spatial resolution ; Micro-fluidic devices ; Multi materials ; Neo-vascularization ; Polyethylene glycol diacrylate ; Vascular endothelial growth factor ; Digital microfluidics
  8. Source: Advanced Materials ; Volume 30, Issue 27 , 2018 ; 09359648 (ISSN)
  9. URL: https://onlinelibrary.wiley.com/doi/abs/10.1002/adma.201800242