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Development of a Paper-based Microfluidic Device for Biological Assay

Boodaghi, Miad | 2018

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 50485 (08)
  4. University: Sharif University of Technology
  5. Department: Mechanical Engineering
  6. Advisor(s): Shamloo, Amir
  7. Abstract:
  8. All the biological diagnostic devices that are introduced to the consumers, must meet WHO criteria. Some of these criteria include being affordable, sensitive and deliverable to the user. In the last twenty years, there have been lots of efforts to use microfluidic devices for biological assay. Due to their expensive price and requirement of complex equipment for their fabrication, polymer-based microfluidic devices have not been able to be used in developing countries. It is to be hoped that introduction of paper for fabrication of microfluidic devices could make microfluidic devices meet WHO criteria. μPADs are divided into well-based and channel-based devices. In the present work, both types of devices were investigated. One of the paramount aspects concerning channel-based devices is to introduce analytical models to study fluid behavior in porous and hydrophilic media of paper. Different models were introduced and accuracy of every model was investigated experimentally and numerically. Results indicate that the assumption of a paper as a combination of pores and annuluses yields more accurate results in comparison with other analytical models. Generally speaking, a three-dimensional random combination of solid pieces yields in a close numerical and experimental results. Effects of hydrophobic walls and evaporation in combination on fluid flow in the paper were studied. Colorimetry was used to measure the concentration of morphine in a well-based device. Results indicate that fitting Hill equation for well intensity as a function of concentration agrees well with experiment. A domain for concentration where logarithm of concentration could be assumed to be a linear function of intensity was introduced
  9. Keywords:
  10. Microfluidic System ; Morphine ; Porous Media ; Paper ; Hydrofilicity ; Colorimetric ; Hydrophobic Boundary

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