Loading...

Design and Fabrication of a Microfluidic Device for Hydrodynamic Label-Free Isolation of Circulating Tumor Cells From Blood

Dorri Giv, Dina | 2017

803 Viewed
  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 49829 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Vosoughi, Manouchehr; Alemzadeh, Iran
  7. Abstract:
  8. Circulating tumor cells (CTCs) are cancer cells which shed from the primary tumor, enter circulation and spread cancer all over the body. CTCs give valuable information about cancer and can help diagnosis and prognosis. Hence separating and isolating CTCs from blood is a necessity for cancer therapeutics. CTCs are separated from blood cells by their different physical or biochemical properties. Many groups have attempted different methods for separation of CTCs. Inertial microfluidics is one of the newest approaches in CTC separation technologies which separates CTCs based on their size. Current inertial microfluidic systems containing straight, spiral, serpentine and contraction expansion arrays (CEA) are designed and optimized by a large number of trial and error experiments while a primary stage simulation can eliminate many of experimental efforts. Here an inertial microfluidic system with a CEA structure is designed with COMSOL and experiments are done based on simulation. Experiments show an 82% separation for a suspension of fixed cells and a 40% separation for cancer cells spiked in diluted blood while separation efficiency is 100% in simulations
  9. Keywords:
  10. Simulation ; COMSOL Software ; Cancer ; Circulating Tumor Cells (CTC) ; Microfluidic System ; Cell Separation ; Metastasis

 Digital Object List

 Bookmark

No TOC