Invetigation Over Bio Elimination of Para Chlorophenol by Paraconiothyrium Fungal Strain Variabile

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Movahednia, Mohammad Mehdi | 2011

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Type of Document: M.Sc. Thesis
Language: Farsi
Document No: 41808 (06)
University: Sharif University of Technology
Department: Chemical and Petroleum Engineering
Advisor(s): Yaghmaei, Soheila; Faramarzi, Mohammad Ali
Abstract:
The ability of Paraconiothyrium variabile, a soil isolated ascomycete, was investigated to remove para chlorophenol in submerged culture media. Among the tested para chlorophenol concentrations, concentration of 40 and 100 mg l-1were found to have minimum and maximum toxic effects, respectively, on the growth of the microorganism and at the same time high and low bioelimination percentages. The fungal strain was able to remove 86% of ρ-CP (with initial concentration of 40 mg l-1) but no significance removal observed at 100 mg l-1 concentration. Monitoring of laccase production level in the fermentation broth together with pollutant removal confirmed the key role of this copper containing oxidase in chlorophenol derivatives elimination.
In order to optimize the bioellemination percentage type of laccase inducer (guaiacol) and its final concentration (250 µM) and also initial pH of the fermentation broth (pH=5.5) in the elimination ρ-CP increased the final removal yield from 86% to 94.3%. Using some modifications in aeration volume yield in complete removal of P-chlorophenol at concentration of 40 mg l-1
Keywords:
Para-Chloro Phenol ; Bioelimination ; Paraconiothyrium Fungi ; Laccas Enzyme

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