Sharif Digital Repository

Bioinspired materials can mimic the stem cell environment and modulate stem cell differentiation and proliferation. In this study, biomimetic micro/nanoenvironments were fabricated by cell-imprinted substrates based on mature human keratinocyte morphological templates. The data obtained from atomic force microscopy and field emission scanning electron microscopy revealed that the keratinocyte-cell-imprinted poly(dimethylsiloxane) casting procedure could imitate the surface morphology of the plasma membrane, ranging from the nanoscale to the macroscale, which may provide the required topographical cell fingerprints to induce differentiation. Gene expression levels of the genes analyzed... 

Chronic, non-healing wounds place a significant burden on patients and healthcare systems, resulting in impaired mobility, limb amputation, or even death. Chronic wounds result from a disruption in the highly orchestrated cascade of events involved in wound closure. Significant advances in our understanding of the pathophysiology of chronic wounds have resulted in the development of drugs designed to target different aspects of the impaired processes. However, the hostility of the wound environment rich in degradative enzymes and its elevated pH, combined with differences in the time scales of different physiological processes involved in tissue regeneration require the use of effective drug... 

In this study, fabrication of a three-dimensional porous scaffold was performed using freeze gelation method. Recently, fabrication of scaffolds using polymer blends has become common for many tissue engineering applications due to their unique tunable properties. In this work, we fabricated alginate-gelatin porous hydrogels for wound healing application using a new method based on some modifications to the freeze-gelation method. Alginate and gelatin were mixed in three different ratios and the resulting solutions underwent freeze gelation to obtain 3D porous matrices. We analyzed the samples using different characterization tests. The scanning electron microscopy (SEM) results indicated... 

Polymer-based composites are used for wound healing applications. This work aims to prepare an inorganic-polymer nanocomposite based on bentonite, poly(vinyl alcohol), and bacterial cellulose by electrospinning for wound healing. The nanocomposite is synthesized using a solution intercalation technique, with 1–2 wt% nanobentonite concentration variation. The effects of commercial and laboratory-synthesized nanobentonite as well as the extract of the green walnut shell (EGWS) are examined and characterized by different techniques. The addition of nanobentonite increases the average size of fibers and tensile strength up to 200 nm and more than 15 MPa, respectively, due to the presence of...