Sharif Digital Repository

Natural tissues of body consist of Extra Cellular Matrix (ECM) and cells. Scaffold produces a temporary matrix for cells and facilitates oxygen and nutrients transport which enhances tissue regeneration. Therefore, attempts in regeneration of tissues and organs by scaffolds attracted great interest in today’s researchs. Choosing scaffold fabrication method and its polymer type, play a key role in scaffold specifications as they determine scaffold properties. In this project electrospinning was chosen as the scaffold fabrication method which is because of the unique properties of scaffolds fabricated by this method and also its ability in producing micro and nano fibers. At first set of... 

This work describes the effect of hydroxyapatite with different morphology including nanorods and whisker on the properties of hydroxyapatite/polycaprolactone (HA/PCL) composite. Biodegradable polymer/Hydroxyapatite (HA) composites with the morphology of nanorods and whisker of (HA) as bone replacement scaffolds are synthesized by sol-gel and hydrothermal methods, respectively with a Ca/P ratio of 1.67 and 20:80 (HA/PCL).The crystallization behavior and porosity of HA/PCL composite are studied by scanning electron microscope and X-Ray diffraction. The response of bone marrow-derived human mesenchymal stem cells (hMSCs) in terms of cell proliferation and differentiation to the osteoblastic... 

Development of new engineered materials for bone tissue engineering applications is rapidly growing. The most important characteristics of materials, which can be used for bone tissue engineering applications, are appropriate mechanical properties, degradation rate, swelling behavior, and bioactivity. The goal of the current investigation is to study the feasibility of incorporating a modified nano-biocomposite based on biodegradable thermoplastic starch for bone tissue engineering. Nano particles of ?-tricalcium phosphate and hydroxyapatite were incorporated for reinforcing the matrix as well as improving the bioactivity. A second biodegradable polymer, i.e. polycaprolactone, was used... 

Hydroxyapatite is the most substantial inorganic constituent of bone tissue which displays splendid biocompability and bioactivity. Nevertheless, its mechanical properties is not utmost appropriate for a bone substitutes. Therefore, it is used to improve the mechanical properties of polymer matrix composite scaffolds. In the present work polycaprolactone as a polymeric matrix was employed to fabricate hydroxyapatite-polycaprolactone biocomposite scaffolds via in situ route. Solvothermal method was employed to synthesize in situ hydroxyapatite in polymer matrix. Porous scaffolds were fabricated via freeze-drying/porogen leaching. Physical, mechanical (compressive module and compressive... 

Synthetic polymers have a high capacity to provide and manufacture the pieces used in tissue engineering. However, optimizing the biocompatibility, bioresorbableility and mechanical properties simultaneous with processability of material is complicated and requires a lot of effort at designing different parts of the system. On the other hand, due to existence of secondary bonds (such as metal-ligand coordination or hydrogen bond) supramolecular polymers are able to respond to some stimuli such as temperature, pH, or pressure, which is important in terms of dynamic nature of interaction between the living cells and the external matrix of cell. Recently there have been extensive studies on... 

Hydroxyapatite (HA) is the most substantial mineral constituent of a bone which displays splendid biocompatibility and bioactivity properties. Nevertheless, its mechanical property is not utmost appropriate for a bone substitution. Therefore, a composite consist of HA and a biodegradable polymer is usually prepared to generate an apt bone scaffold. In the present work polycaprolactone (PCL), a newly remarkable biocompatible and biodegradable polymer, was employed as a matrix and hydroxyapatite nanoparticles and nanorods were used as a reinforcement element of the composite. HA/PCL nanocomposites were synthesized by three new in-situ sol-gel processes using a diverse range of phosphorus and... 

Combined composites consisting of nanofibers and hydrogels were fabricated to grow cells in an environment that mimicked the extracellular matrix. Electrospan nanofibers were spun and hydrolyzed under optimized conditions consisting of polycaprolactone (PCL) and gelatin and then incorporated into alginate and gelatin hydrogels. Nanofibril-containing (NF / hydrogel) hydrogel hybrids were ligated by calcium chloride in the presence of encapsulated beta cells. The mechanical properties of NF / hydrogel scaffolds were significantly increased in proportion to the NF content in NF / hydrogels. Beta cells killed in NF / hydrogels showed higher adhesion to the matrix compared to samples without NF.... 

Polycaprolactone (PCL) scaffolds and its composites containing bioactive glass particles (45S5) and TiO2 nanostructures with pre-defined and controlled external and internal architecture were prepared via an indirect three-dimensional (3D) printing process. The scaffolds had an interconnected structure with macro- (400-500 μm) and micro- (~25 μm) pores. Bioactivity, mechanical behavior and kinetics of tissue growth in 3D scaffolds were studied. The size effect of biogactive glass particles (6 μm, 250 nm, <100 nm) and morphology of titania nanostructures (spherical, tube, leaf-like, and flower-like particles) were elaborated. The biogactive glass particles with different sizes were prepared... 

The aim of this study is the fabrication of 3D porous PCL scaffolds contain core-shell fibers for cartilage tissue engineering. The novel fabrication method is co-axial wet electrospinning simultaneously. These tablets like scaffolds have superior porosity and pore sizes for cell culturing and cell-cell interaction as they have good mechanical properties for cartilage tissue engineering in comparison to 2D electrospun scaffolds. The structure of these 3D scaffolds is mimicking the ECM of cartilage. This study presents the coaxial electrospinning of PCL nanofibers encapsulated with bovine serum albumin and platelet rich plasma for demonstration of controlled release and bioactivity retention,... 

Shape memory polymers have the potential to use in the field of biomedical applications. In this research, polyurethane structures have been synthesized based on the Diol mixtures, consisting of polycaprolactane diol (PCL-diol) and poly (lactic acid) diol (PLA-diol). Aim of reasearch is about evaluating poly (lactic acid) effect in structure of the polyurethane. At the same time, cellulose nanocrystals (CNC) have been added to polymer matrix to improve properties. The synthesis of acrylated polyurethane, included the synthesis of polyurethane acrylate prepolymer. Next, this prepolymer has been cured by adding the reactive diluent and some polyol. The cured systems were investigated, finally,... 

Today, current methods are used for treatment of cancers. Patient experiences a period of hard treatment during destruction of both healthy and diseased cells. Researchers are following stimulus- response drug delivery systems that they control time and position of drug release. Meanwhile, micellar nanoparticles are important, because it can transport the hydrophobic anticancer drugs from body's physiological barriers. On the other hand, tumour tissue has low pH and high temperature as compared to health tissue. Therefore, using pH and thermosensitive polymer coating could be effective in triggered drug release. In the first study, micellar systems were synthesized which were responsive to... 

Smart bio-based shape memory polymers with high performance and fast response have the exciting potential to meet the growing need in biomedical applications. A fascinating approach in SMP categories in biomedical field is the development of noncontact activation without increasing the environmental temperature through remote triggering methods, such as magnetic triggering. Magnetically responsive SMPs have received lots of attention in the biomedical applications, especially the non-contact triggering of medical equipment and implants inside the body. In this study, the biocompatible nanocomposite based on shape memory polyurethane acrylates (SMPUAs)/ hybrid carbon-magnetic nanoparticles,... 

Craniofacial bone defects without scarring are a major clinical issue. These critical defects, which can be caused by infection or fracture, cannot be repaired without surgery. The main goal of skull repair is to protect vulnerable structures such as the brain, or it can be due to the improvement of jaw function and beauty. Tissue engineering can offer a new generation solution. The purpose of this study was to construct and characterise a degradable polymer/ bioceramic composite scaffold with allograft powder used in the craniofacial bone. The complexities of the extracellular matrix of humans can be simulated using 3D bioprinting.In this study, tricalcium silicate (TCS) was first... 

Bio-based shape memory polymers with fast-response have high potential for use in medical applications. In this study, biocompatible and biodegradable nanocomposites based on shape memory polyurethane acrylates (SMPUA)/cellulose nanocrystals (CNC), were synthesized by in-situ polymerization. CNC is a natural and renewable nanoparticle that has been considered for medical applications due to its excellent mechanical and biological properties and surface chemistry. At the first phase of this study, in order to synthesize UV-curable shape memory polyurethane acrylate, polycaprolactone diol (PCL-Diol), hexamethylene diisocyanate (HDI) and hydroxyethyl methacrylate (HEMA) were used as an reactive...