Loading...
Search for:
osteogenic
0.009 seconds
Human olfactory mucosa stem cells delivery using a collagen hydrogel: As a potential candidate for bone tissue engineering
, Article Materials ; Volume 14, Issue 14 , 2021 ; 19961944 (ISSN) ; Milan, P. B ; Saadatmand, M ; Bagher, Z ; Gholipourmalekabadi, M ; Alizadeh, R ; Hivechi, A ; Arabpour, Z ; Hamidi, M ; Delattre, C ; Sharif University of Technology
MDPI AG
2021
Abstract
For bone tissue engineering, stem cell‐based therapy has become a promising option. Re-cently, cell transplantation supported by polymeric carriers has been increasingly evaluated. Herein, we encapsulated human olfactory ectomesenchymal stem cells (OE‐MSC) in the collagen hydrogel system, and their osteogenic potential was assessed in vitro and in vivo conditions. Col-lagen type I was composed of four different concentrations of (4 mg/mL, 5 mg/mL, 6 mg/mL, 7 mg/mL). SDS‐Page, FTIR, rheologic test, resazurin assay, live/dead assay, and SEM were used to characterize collagen hydrogels. OE‐MSCs encapsulated in the optimum concentration of collagen hydrogel and transplanted in rat calvarial...
Graphene nanogrids for selective and fast osteogenic differentiation of human mesenchymal stem cells
, Article Carbon ; Volume 59 , 2013 , Pages 200-211 ; 00086223 (ISSN) ; Ghaderi, E ; Shahsavar, M ; Sharif University of Technology
2013
Abstract
Graphene nanogrids (fabricated by graphene nanoribbons obtained through oxidative unzipping of multi-walled carbon nanotubes) were used as two-dimensional selective templates for accelerated differentiation of human mesenchymal stem cells (hMSCs), isolated from umbilical cord blood, into osteogenic lineage. The biocompatible and hydrophilic graphene nanogrids showed high actin cytoskeleton proliferations coinciding with patterns of the nanogrids. The amounts of proliferations were found slightly better than proliferation on hydrophilic graphene oxide (GO) sheets, and significantly higher than non-uniform proliferations on hydrophobic reduced graphene oxide (rGO) sheets and...
In vitro bactericidal and drug release properties of vancomycin-amino surface functionalized bioactive glass nanoparticles
, Article Materials Chemistry and Physics ; Volume 241 , 2020 ; Ghorbani, M ; Bagheri, K.P ; Shokrgozar, M. A ; Sharif University of Technology
Elsevier Ltd
2020
Abstract
Bioactive glass has been used clinically in bone repair applications for bone grafting because of its prominent physiochemical and osteogenic properties. Various attempts have been made to enhance bioactive glass efficiency in combination with other biomaterials such as antibiotics and growth factors. In present study, we developed a modification of bioactive glass nanoparticles that insured long term antibacterial effect. Bioactive glass nanoparticles (BGNs) were functionalized with (3-Aminopropyl) triethoxysilane (APTS), then vancomycin (VAN) was immobilized onto BGNs-APTS via EDC/NHS cross-linking process. Another study group namely BGNs-VAN was synthesized as a control group without any...
Computational modeling of media flow through perfusion-based bioreactors for bone tissue engineering
, Article Proceedings of the Institution of Mechanical Engineers, Part H: Journal of Engineering in Medicine ; Volume 234, Issue 12 , 2020 , Pages 1397-1408 ; Bohlouli, M ; Adavi, K ; Paknejad, Z ; Rezai Rad, M ; khani, M. M ; Salehi-Nik, N ; Khojasteh, A ; Sharif University of Technology
SAGE Publications Ltd
2020
Abstract
Bioreactor system has been used in bone tissue engineering in order to simulate dynamic nature of bone tissue environments. Perfusion bioreactors have been reported as the most efficient types of shear-loading bioreactor. Also, combination of forces, such as rotation plus perfusion, has been reported to enhance cell growth and osteogenic differentiation. Mathematical modeling using sophisticated infrastructure processes could be helpful and streamline the development of functional grafts by estimating and defining an effective range of bioreactor settings for better augmentation of tissue engineering. This study is aimed to conduct computational modeling for newly designed bioreactors in...