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Covalent immobilization of cellulase using magnetic poly(ionic liquid) support: improvement of the enzyme activity and stability
, Article Journal of Agricultural and Food Chemistry ; Volume 66, Issue 4 , 2018 , Pages 789-798 ; 00218561 (ISSN) ; Hosseini, A ; Zohreh, N ; Yaghoubi, M ; Pourjavadi, A ; Sharif University of Technology
American Chemical Society
2018
Abstract
A magnetic nanocomposite was prepared by entrapment of Fe3O4 nanoparticles into the cross-linked ionic liquid/epoxy type polymer. The resulting support was used for covalent immobilization of cellulase through the reaction with epoxy groups. The ionic surface of the support improved the adsorption of enzyme, and a large amount of enzyme (106.1 mg/g) was loaded onto the support surface. The effect of the presence of ionic monomer and covalent binding of enzyme was also investigated. The structure of support was characterized by various instruments such as FT-IR, TGA, VSM, XRD, TEM, SEM, and DLS. The activity and stability of immobilized cellulase were investigated in the prepared support. The...
Immobilization of laccase from trametes hirsuta onto CMC coated magnetic nanoparticles
, Article International Journal of Engineering, Transactions A: Basics ; Volume 33, Issue 4 , 2020 , Pages 513-519 ; Ghazvini, S ; Hejazi, S ; Yaghmaei, S ; Ghobadi Nejad, Z ; Sharif University of Technology
Materials and Energy Research Center
2020
Abstract
In this study Fe3O4/CMC magnetic nanoparticles were synthesized through co-precipitation method. Afterward, laccase from Trametes hirsuta was immobilized onto Carboxymethyl cellulose (CMC)-coated magnetic Fe3O4 nanoparticles by covalent bonding between carboxyl groups of carboxymethyl cellulose and amine group of laccases. Also, the resulted magnetic nanoparticles and immobilized laccase were characterized by Fourier-transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) and dynamic light scattering (DLS) analysis. Moreover, the vital factors in enzyme immobilization, such as contact time, amount of N-hydroxysuccinimide (NHS), and the amount of nanoparticles were...