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horseradish-peroxidase-enzymes
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Two nanostructured polymers: Polyaniline nanofibers and new linear-dendritic matrix of poly(citric acid)-block-poly(ethylene glycol) copolymers for environmental monitoring in novel biosensors
, Article International Journal of Polymeric Materials and Polymeric Biomaterials ; Volume 62, Issue 7 , Jul , 2013 , Pages 377-383 ; 00914037 (ISSN) ; Vossoughi, M ; Alemzadeh, I ; Naeini, A. T ; Darvish, M ; Sharif University of Technology
2013
Abstract
In this work two phenol biosensors, one based on polyaniline nanofibers (PNFs) and the other based on the newly created and introduced linear-dendritic matrix of poly(citric acid)-block-poly(ethylene glycol) copolymers (PCA-PEG-PCA), were chemically modified with horseradish peroxidase (HRP) enzyme. These phenol biosensors showed an oxidation peak at 0.55 V. The amperometric response for biosensors based on PNFs showed a linear response range from 2.5 × 10-6 to 2.5 × 10-5 mol/L, with a detection limit of 2.5 M phenol. Also, the amperometric response for a biosensor based on PCA-PEG-PCA showed a linear response range from 2.5 × 10-6 to 4 × 10-5 mol/L, with a detection limit of 1.5 M phenol
Nano reengineering of horseradish peroxidase with dendritic macromolecules for stability enhancement
, Article Enzyme and Microbial Technology ; Volume 50, Issue 1 , 2012 , Pages 10-16 ; 01410229 (ISSN) ; Vossoughi, M ; Shahrokhian, S ; Alemzadeh, I ; Sharif University of Technology
Abstract
A simple bio-conjugation procedure to surround a single horseradish peroxidase (HRP) enzyme molecule with dendritic polyester macromolecules (polyester-32-hydroxyl-1-carboxyl bis-MPA dendron, generation 5) was proposed. The characterization of resultant nanoparticles entitled HRP dendrozyme, was performed by transmission electron microscopy, dynamic light scattering, gel permeation chromatography and Fourier transform infrared spectroscopy. The results showed that HRP nanoparticles were spherical in shape and have an average size of 14 ± 2. nm in diameter. Furthermore, bio-conformational characterization of HRP dendrozyme was performed by means of circular dichroism and fluorescence...