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Fabrication and characterization of electrospun poly-L-lactide/gelatin graded tubular scaffolds: Toward a new design for performance enhancement in vascular tissue engineering
, Article Progress in Natural Science: Materials International ; Volume 25, Issue 5 , October , 2015 , Pages 405–413 ; 10020071 (ISSN) ; Tahmasbi, M ; Amoabediny, G ; Nourmohammadi, J ; Moztarzadeh, F ; Mozafari, M ; Sharif University of Technology
Elsevier
2015
Abstract
In this study, a new design of graded tubular scaffolds have been developed for the performance enhancement in vascular tissue engineering. The graded poly-L-lactide (PLLA) and gelatin fibrous scaffolds produced by electrospining were then characterized. The morphology, degradability, porosity, pore size and mechanical properties of four tubular scaffolds (graded PLLA/gelatin, layered PLLA/gelatin, PLLA and gelatin scaffolds) have been investigated. The tensile tests demonstrated that the mechanical strength and also the estimated burst pressure of the graded scaffolds were significantly increased in comparison with the layered and gelatin scaffolds. This new design, resulting in an increase...
Design and fabrication of conductive nanofibrous scaffolds for neural tissue engineering: Process modeling via response surface methodology
, Article Journal of biomaterials applications ; Volume 33, Issue 5 , 2018 , Pages 619-629 ; 15308022 (ISSN) ; Mashayekhan, S ; Baniasadi, H ; Ramazani, A ; Ansarizadeh, M ; Sharif University of Technology
NLM (Medline)
2018
Abstract
Peripheral nervous system in contrary to central one has the potential for regeneration, but its regrowth requires proper environmental conditions and supporting growth factors. The aim of this study is to design and fabricate a conductive polyaniline/graphene nanoparticles incorporated gelatin nanofibrous scaffolds suitable for peripheral nervous system regeneration. The scaffolds were fabricated with electrospinning and the fabrication process was designed with Design-Expert software via response surface methodology. The effect of process parameters including applied voltage (kV), syringe pump flow rate (cm3/h), and PAG concentration (wt%), on the scaffold conductivity, nanofibers diameter,...
Survivability and oxidative stability of co-microencapsulated L. Plantarum PTCC 1058 and DHA as a juice carrier
, Article Food Bioscience ; Volume 32 , 2019 ; 22124292 (ISSN) ; Alemzadeh, I ; Vossoughi, M ; Sharif University of Technology
Elsevier Ltd
2019
Abstract
Lactobacillus Plantarum PTCC 1058 bacteria and DHA-rich oil were microencapsulated together or individually in an optimized alginate-pectin-gelatin biocomposite hydrogel. The survivability of L. plantarum and oxidative stability of DHA were evaluated using simulated gastrointestinal (SGI) conditions and 4 wk storage in commercial orange juice at 4 and 25 °C. The results showed that the multilayer biocomposite had significant influence on the stability of the microcapsules in terms of survivability of probiotic bacteria and oxidative stability of DHA with SGI conditions and storage at 4 and 25 °C compared to the free cells. Incorporation of probiotic bacteria and DHA showed the synergistic...
Gelatin-based functional films integrated with grapefruit seed extract and TiO2 for active food packaging applications
, Article Food Hydrocolloids ; Volume 112 , 2021 ; 0268005X (ISSN) ; Priyadarshi, R ; Rhim, J. W ; Bagheri, R ; Sharif University of Technology
Elsevier B.V
2021
Abstract
Gelatin-based functional films were prepared by the addition of grapefruit seed extract (GSE, 5 wt% based on gelatin) and various amounts of TiO2 (0.5, 1.0, 3.0, and 5.0 wt% based on gelatin). TiO2 was evenly dispersed in the gelatin film, but the film surface roughness was increased as the concentration of TiO2 increased. The mechanical strength and water contact angle (WCA) of the composite film were the highest, while the water vapor permeability (WVP) was the lowest when 0.5 wt% TiO2 was used. The addition of GSE slightly reduced the UV light transmittance, but the addition of TiO2 almost completely prevented the UV light transmission. The addition of GSE and TiO2 did not significantly...
Synthesis and Investigation of the Swelling Behavior of Superabsorbents Based on Gelatin and k-Carrageenan and Loading of KNO3 as aModel of Agrochemical Onto Them
, M.Sc. Thesis Sharif University of Technology ; Pourjavadi , Ali (Supervisor)
Abstract
This thesis focuses on the preparation and super-swelling behavior of a superabsorbent hydrogel based on natural macro-molecules and loading potassium nitrite as an agro-chemical onto it. 1-In this investigation a new type of superabsorbent hydrogel based on Gelatin and k-Carrageenan was prepared and the effect of the feed ratio of some components (acrylic acid, MBA, APS, and agar) on the swelling capacity of the hydrogel was systematically studied. The structure of the hydrogel optimized final product was found to be 1064 g/g in distilled water. The structure of the hydrogel was characterized by FT_IR method and morphology of the samples was examined by scamming electron microscopy (SEM)....
Preparation of Gelatin-Alginate Hydrogel for Using as Cell Culture Scaffold
, M.Sc. Thesis Sharif University of Technology ; Mashayekhan, Shohreh (Supervisor) ; Maghsoudi, Vida (Supervisor)
Abstract
Hydrogels are polymeric three-dimensional networks able to swell in the presence of an aqueous medium. Hydrogels from natural proteins and polysaccharides are ideal scaffolds for tissue engineering since they resemble the extracellular matrices of tissue comprised of various amino acids and sugar-based macromolecules.The biocompatible and biodegradable hydrogel scaffolds are promising materials for tissue engineering. Here, we report a new class of hydrogels derived from oxidized alginate (OA) and gelatin. The prepared oxidized alginate was shown to be efficient in crosslinking gelatin, leading to hydrogel formation. The effect of degree of oxidation and concentration of OA on the mechanical...
Investigating the Optimum Conditions for Cell Growth and Behavior on Hydrogel Surfaces
, M.Sc. Thesis Sharif University of Technology ; Mashayekhan, Shohre (Supervisor) ; Maghsudi, Vida (Supervisor)
Abstract
Generally, the concept of producing ‘spare parts’ of the body for replacement of damaged or lost organs lies at the core of the varied biotechnological practices referred as tissue engineering. Tissue engineering is an interdisciplinary field that incorporates principles of engineering with the life sciences. Tissue engineering is based on three principle; cells, scaffolds for cells expansion, attachment as an environment like ECM and growth factors. These things together can help tissue engineers to provide microenvironments which are suitable for special cells. The most important thing in this kind of works is the ability to simulate environment for cells the same as body. According to the...
Chitosan/Gelatin/Mg Substituted Hydroxyapatite Nano Composite Scaffold for Bone Tissue Engineering
, M.Sc. Thesis Sharif University of Technology ; Mohammadi, Mohammad Reza (Supervisor) ; Fathi, Mohammad Hossein (Co-Advisor)
Abstract
The present study is work on fabrication and characterization of a chitosan/ gelatin/ Mg substituted hydroxyapatite nano composite scaffold for bone tissue engineering. At the first step pure and Mg-substituted hydroxyapatite (HA) (Ca10-xMgx(PO4) 6OH2) nano-hexagonal rods with 14–45 nmdiameter.for this, calcium nitrate, magnesium phosphate hydrate and potassium dihydrogenphosphate were used as precursors for Ca, Mg, and P, respectively. Calculated amounts of magnesium ions (Mg+2) especially from 0 to 8% (molar ratio) were incorporated as substituted into the calcium sol solution. Deionized water was used as a diluting media for HA sol preparation and ammonia was used to adjust the pH= 9....
Biopolymers Mediated Green Synthesis of Silver Nanoparticles With Definite Shape and Their Biomedical Activity Investigation
, M.Sc. Thesis Sharif University of Technology ; Pourjavadi, Ali (Supervisor)
Abstract
Silver nanoparticles (Ag-NPs) are promising agents in bionanotechnology, because of their unique activity against unfavorable processes in bioscience such as undesirable microbial growth, angiogenesis in tumors and etc. Ag-NPs have been widely used in wound dressing; wound healing, water treatment filters, inks, sensors, catalysts, cosmetics, orthopaedics, surgical instruments and vascular prosthesis. Ever-increasing applications of Ag-NPs in biomedical field are due to the anti-bacterial, anti-fungal, anti-viral, anti-platelet, anti-proliferative, anti-inflammatory (apoptosis detection) and anti-angiogenesis properties (cancer therapy). In the current research, we synthesized Ag-NPs in...
Extraction and processing optimization of gelatin from fish (otolithes ruber) skin
, Article CHISA 2006 - 17th International Congress of Chemical and Process Engineering, Prague, 27 August 2006 through 31 August 2006 ; 2006 ; 8086059456 (ISBN); 9788086059457 (ISBN) ; Aminshahidi, B ; Meraj, F ; Sharif University of Technology
2006
Abstract
Gelatin is a denatured protein derived from collagen by thermohydrolysis and has a rheological property of thermo reversible transformation between sol & gel. Gelatin is one of the most important biopolymers, with widespread applications in the food, pharmaceutical, cosmetic and photographic industries. Gelatin is commercially made from skins and skeletons of bovine and porcine and mammalian gelatin has been intensively studied. However, mammalian gelatin is at risk for contamination with bovine spongiform encephalopathy and increase in demand for kosher and halal foods have created a demand for fish gelatin for food applications. Therefore, the objective of this study was to extract gelatin...
Sol-Gel Preparation and Study of Bio-Composite Scaffold Containing Chitosan-Gelatin/ Nano-Hydroxyapatite for Bone Tissue Engineering
, M.Sc. Thesis Sharif University of Technology ; Mohammadi, Mohammad Reza (Supervisor)
Abstract
In present work, hydroxyapatite nano-rods are synthesized using a simple sol-gel method with calcium nitrate and potassium dihydrogenphosphate as calcium and phosphorus precursors respectively and ammonia was used for adjusting the pH=9. To obtain rod shape hydroxyapaite powder, multi wall carbon nanotubes were added to the solution, nHA powder was calcinated at 60 and 450 ℃. In second step, chitosan-gelatin/nanoHA solution were prepared by blending chitosan and gelatin in different concentrations with nanoHA powder, then the solution was freeze dried to maintain a composite scaffold. The prepared HA powder and bio-composite scaffold were characterized by X-ray diffraction (XRD), field...
Design and Fabrication of Hydrogel Microcarrier for Tissue Engineering
, M.Sc. Thesis Sharif University of Technology ; Mashayekhan, Shohre (Supervisor) ; Yaghmaei, Soheila (Supervisor)
Abstract
Microcarriers (MCs) are an attractive technology with various applications in tissue engineering. In this study, chitosan/gelatin MCs were fabricated with blends of different ratio of chitosan/gelatin, by using a setup containing high voltage electrostatic field and syringe pump. Optimization of blend ratio, voltage and syringe pump flow carried out by the “Design expert” software leads to fabrication of MCs with constant diameter while having various elasticity. Mechanical strength and elasticity of MCs were determined. Human umbilical cord mesenchymal stem cells (hUCMSCs) were cultured on MCs dynamically by using mini-rocher in an incubator. Cells adhesion on MCs were successfully shown by...
Mint Flavor Immobilization and Properties Determination
, M.Sc. Thesis Sharif University of Technology ; Alemzadeh, Iran (Supervisor) ; Kazemi, Akhtarelmolook (Supervisor)
Abstract
Peppermint oil is one of the most often used food ingredients and it is volatile. Many common procedures such as microencapsulation have been used for protecting volatile food ingredients. For this research, L-menthol was chosen as the core material to be encapsulated. L-menthol is responsible for the minty flavor and cooling sensation of food and pharmaceutical products. The encapsulation of L-menthol was prepared by complex coacervation, using gum Arabic and gelatin as wall material and tannic acid as cross-linking agent. The effect of various processing parameters, including wall material, core material, tannic acid and tween¬80 concentrations, on particle size and efficiency was...
Synthesis of γ-Fe2O3 Nanoparticles by Gelatin Mediator and Synthesis of γ-Fe2O3/SiO2/PCA/Ag-NPs Nanostructure Drug Delivery System
, M.Sc. Thesis Sharif University of Technology ; Pourjavadi, Ali (Supervisor)
Abstract
Maghemite (γ-Fe2O3) nanoparticles were produced after high temperature oxidation of gelatin-Fe3O4 nanocomposite, obtained from a common coprecipitation process of Fe2+ and Fe3+ ions in an alkaline medium in the presence of a gelatin protein as an effective mediator. Size, shape, surface morphology and magnetic properties of the prepared γ-Fe2O3 nanoparticles were characterized using XRD, FTIR, TEM, SEM and VSM data. The results revealed that primary, gelatin-Fe3O4 nanocomposite had been formed and next, the gelatin decomposition in furnace produced a spinel structure of γ-Fe2O3. The effects of furnace temperature and time of heating together with the amount of gelatin on the produced...
Construction of Scaffold by Electrospinning Method for Use in Skin Tissue Engineering
, M.Sc. Thesis Sharif University of Technology ; Alemzadeh, Iran (Supervisor) ; Vosoughi, Manouchehr (Supervisor)
Abstract
Making artificial skin and skin alternatives is one of the most important areas in tissue engineering. Although much progress has been made so far, there is still no definitive cure for second- and third-degree burns. To create new tissue in the body, a suitable substrate for cells is needed, which is called scaffolding, and an ideal scaffold in tissue engineering should mimic the dimensions of extracellular matrix, and nanofibers seem to be the best option for this purpose. Among the methods of manufacturing of nanofibers, electrospun is very easy and accurate method. In previous studies, many natural and synthetic polymers such as chitosan, alginate, collagen, polyathylene oxide, etc....
Encapsulation of peppermint oil with arabic gum-gelatin by complex coacervation method
, Article International Journal of Engineering, Transactions B: Applications ; Volume 26, Issue 8 , 2013 , Pages 807-814 ; 10252495 (ISSN) ; Alemzadeh, I ; Kazemi, A ; Sharif University of Technology
2013
Abstract
The gelatin/gum arabic microcapsules encapsulating peppermint oil were prepared by complex coacervationusing tannic acid as hardening agent. The effects of various parameters, including concentration of wall material, core material, tannic acid and Tween 80 were investigated on particle size and encapsulation efficiency. For statistical evaluation of the parameters, Taguchi method was concerned. The size of prepared spherical microcapsules was 19-66 micrometers. The results showed that, particle size increased with increasing the core and the wall concentration and decreased with increasing tannic acid and Tween 80 concentraIfd efficiency increased by increasing the core and wall...
Preparation of Gelatin/Pva Porous Scaffolds by Chemical and Gamma Irradiation Methods for Skin Cell Culture
, M.Sc. Thesis Sharif University of Technology ; Frounchi, Masoud (Supervisor) ; Dadbin, Susan (Supervisor)
Abstract
The goal of this research project was to prepare gelatin/PVA porous scaffolds by chemical and gamma irradiation methods to be used for human dermal fibroblast cell culture. First, the polymers were dissolved in hot water and mixed properly to achieve a homogenous solution. The mixture was then frozen at -200C and immersed in ethanol/ glutaraldehyde solution at -200C for 48 hours in order to extract ice crystals and also form chemical crosslinks in gelatin by glutaraldehyde. After being rinsed with distilled water the samples were lyophilized. The effects of polymers solution concentration, gelatin/PVA ratio and glutaraldehyde /gelatin ratio were investigated on characteristics of the...
Hydrolyzed Protein-Based Highly Porous Hydrogels: Synthesis and Characteristics
, Ph.D. Dissertation Sharif University of Technology ; Pourjavadi, Ali (Supervisor)
Abstract
This thesis focuses on the preparation and super-swelling behavior of four highly porous hydrogels: 1. Firstly, we have developed a collagen-based highly porous hydrogel by neutralizing the grafted poly(acrylamide-co-acrylic acid) after gel formation. Systematically, the certain variables of the graft copolymerization were optimized to achieve maximum swelling capacity. Morphology of the samples was examined by scanning electron microscopy (SEM) and was shown the pores were induced into the hydrogel by water evaporation resulting from neutralization process. The absorbency under load (AUL) and centrifuge retention capacity (CRC) were measured. The synthesized hydrogel exhibited a...
Design and Manufacture of an Engineered Scaffold in Order to Repair Tissue Exposed to Mechanical Force, for Tissue Engineering Application
, M.Sc. Thesis Sharif University of Technology ; Shamloo, Amir (Supervisor)
Abstract
Articular cartilage has limited repair capability following traumatic injuries and current methods of treatment remain inefficient. Reconstructing cartilage provides a new way for cartilage repair and natural polymers are often used as scaffold because of their biocompatibility and biofunctionality. The goal of cartilage tissue engineering is to design a scaffold with proper pore structure and similar mechanical properties to the native tissue. In this study Porous scaffolds prepared from silk fibroin, chitosan and gelatin blends with varying ratio of silk fibroin and gelatin by freeze drying method were characterized for physicochemical, mechanical and biological properties. Among the...