Sharif Digital Repository

Bioorthogonal chemistry defines any reaction, inside or outside the living systems, without interfering with native chemical processes and 3dimensional structure of proteins. In these types of reactions water is the sole solvent; a neutral pH is required; the temperature must be up to 40 oC. The kinetics of reactions must be on the hour scale and the nontoxic reagents with low concentration must be used. One of the simplest methods of visualizing protein molecule is covalent attachment of fluorescein to protein. In this work several fluorescent compounds were synthesized. These novel compounds include acylchloride fluorescein, N-hydroxysuccinimide fluorescein, thiophenol fluorescein, and...