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    Increased Expression of Flagellins Recombinant Soluble form by Chemical Chaperon

    , M.Sc. Thesis Sharif University of Technology Bakhtiarvand, Bahador (Author) ; Yaghmaei, Soheila (Supervisor) ; Tarahomjoo, Shirin (Supervisor)
    Abstract
    Flagella is the organelle involved in motility of Salmonella and plays an important role in colonization of Salmonella. Flagellins is the structural subunits of flagellar filament that is known as an adjuvant. Recombinant production of flagellin of S. enteritidis is advantageous due to the removal of pathogenic microorganism from the production process and improved process safety. E.coli is an appropriate host for the expression of recombinant proteins. However, its major obstacle is the formation of inclusion bodies. Inclusion bodies are aggregated inactive proteins and the recovery of proteins in the active form from these aggregates usually has a low yield. Therefore, the objective of our... 

    Planar Motion Control and Path Following of a Biomimetic Helical Swimmer Robot in Low Reynolds Condition

    , M.Sc. Thesis Sharif University of Technology Ghasemi, Mahdi (Author) ; Sayadi, Hassan (Supervisor)
    Abstract
    Today, the use of controllable swimmer micro-robots has expanded from micro to macro-scale dimensions due to their many applications. These robots, as a result of their small size and dimensions, have the ability to access limited and complex environments. Considering the various applications of such swimmers, it can be said that in addition to the necessity of well designing and implementation, their maneuverability and controllability are among the most important research areas on these swimmers. Regarding the dimensions and technologies needed for these robots, various methods for designing and implementing them are suggested, which are mostly inspired by the nature of microorganisms. In... 

    Chaperones promote remarkable solubilization of salmonella enterica serovar enteritidis flagellin expressed in escherichia coli

    , Article Protein and Peptide Letters ; Volume 27, Issue 3 , 2020 , Pages 210-218 Bakhtiarvand, B ; Sadeghi, Z ; Tarahomjoo, S ; Yaghmaie, S ; Sharif University of Technology
    Bentham Science Publishers  2020
    Abstract
    Background: Flagellin of Salmonella enterica serovar Enteritidis (SEF) stimulates immune responses to both itself and coapplied antigens. It is therefore used in vaccine development and immunotherapy. Removal of pathogenic S. enterica ser. Enteritidis from SEF production process is advantageous due to the process safety improvement. The protein solubility analysis using SDS-PAGE indicated that 53.49% of SEF expressed in Escherichia coli formed inclusion bodies. However, the protein recovery from inclusion bodies requires a complex process with a low yield. Objective: We thus aim to study possibility of enhancing SEF expression in E. coli in soluble form using chemical and molecular...