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Nanoporous gold as a suitable substrate for preparation of a new sensitive electrochemical aptasensor for detection of salmonella typhimurium
, Article Sensors and Actuators, B: Chemical ; Volume 255 , 2018 , Pages 1536-1544 ; 09254005 (ISSN) ; Shahrokhian, S ; Nurmohammadi, F ; Sharif University of Technology
Elsevier B.V
2018
Abstract
Nowadays, achievement to easy, fast, reliable and cost-effective techniques and tools is one of the most important challenges in detection of microorganisms. Salmonella typhimurium is one of the most important pathogenic bacteria that affect the human health and environmental infections. In this work we report a new, stable, biocompatible and cost-effective platform for construction of an aptasensor based on nanoporous gold (NPG) for detection of S. typhimurium. Nanoporous gold is electrochemically synthesized using Au-Cu alloy at the surface of Au/GCE. Thiol functionalized aptamer is used for effective linking to the surface of NPG/Au/GCE via self-assemble monolayers (SAMs) formation....