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Cationic graphene oxide nanoplatform mediates miR-101 delivery to promote apoptosis by regulating autophagy and stress
, Article International journal of nanomedicine ; Volume 13 , 2018 , Pages 5865-5886 ; 11782013 (ISSN) ; Akhavan, O ; Mottaghitalab, F ; Adeli, M ; Dinarvand, R ; Razzazan, S ; Arefian, E ; Soleimani, M ; Atyabi, F ; Sharif University of Technology
NLM (Medline)
2018
Abstract
Introduction: MicroRNA-101 (miR-101) is an intense cancer suppressor with special algorithm to target a wide range of pathways and genes which indicates the ability to regulate apoptosis, cellular stress, metastasis, autophagy, and tumor growth. Silencing of some genes such as Stathmin1 with miR-101 can be interpreted as apoptotic accelerator and autophagy suppressor. It is hypothesized that hybrid microRNA (miRNA) delivery structures based on cationized graphene oxide (GO) could take superiority of targeting and photothermal therapy to suppress the cancer cells
Selective Colorimetric Detection of Pentaerythritol Tetranitrate (PETN) Using Arginine-mediated Aggregation of Gold Nanoparticles
, M.Sc. Thesis Sharif University of Technology ; Hormozinezhad, Mohammad Reza (Supervisor)
Abstract
Detection of pentaerythritol tetranitrate (PETN) as an explosive has been of great interest because of public safety and military concerns. Here, we have presented a simple, selective and sensitive colorimetric method for direct detection of PETN. The gold nanoparticles (AuNPs) were first exposed to arginine which has primary amines in its structure. Primary amines from arginine as electron donors could strongly interact with electron deficient –NO2 group of PETN. Also, hydrogen bonding could happen between the –NO2 group of PETN and –NH2 group of arginine molecules. Therefore, selective aggregation of AuNPs happened because of the donor-acceptor and hydrogen bonding interactions. Due to the...
Spectrophotometric Determination of Arginine by Aggregation of Spherical Gold Nanoparticles
, M.Sc. Thesis Sharif University of Technology ; Hormozinezhad, Mohammad Reza (Supervisor)
Abstract
Spectrophotometric determination of arginine by aggregation of spherical Au nanoparticles Arginine has been classified as a ‘semi-essential’ or ‘conditionally-essential’ amino acid. This amino acid plays a crucial role in many biological path ways. Hence, its determination in biological body fluids such as blood plasma and urine is important for clinical diagnosis of various diseases. Herein we report the development of a sensitive colorimetric method for the determination of arginine based on aggregation of the citrate capped gold nanoparticles (Au NPs). Reaction contains replacement of citrate ions with guanidinium groups of arginine followed by establishing a chemical bond between amino...
Graphene oxide-l-arginine nanogel: A pH-sensitive fluorouracil nanocarrier
, Article Biotechnology and Applied Biochemistry ; Volume 66, Issue 5 , 2019 , Pages 772-780 ; 08854513 (ISSN) ; Ghaemi, A ; Masoudi, G ; Chogan, F ; Rashedi, H ; Yazdian, F ; Omidi, M ; Javadi, S ; Haghiralsadat, B. F ; Teimouri, M ; Faal Hamedani, N ; Sharif University of Technology
Wiley-Blackwell Publishing Ltd
2019
Abstract
Nowadays, putting forward an accurate cancer therapy method with minimal side effects is an important topic of research. Nanostructures, for their ability in controlled and targeted drug release on specific cells, are critical materials in this field. In this study, a pH-sensitive graphene oxide-l-arginine nanogel was synthesized to carry and release 5-fluorouracil. Optimized conditions using statistical analysis, based on the maximum relative viscosity of nanogel, were evaluated: 5.489 for the concentration of l-arginine and 2.404 for pH. The prepared nanogels were characterized using scanning electron microscope and transmission electron microscope images and Fourier-transform infrared...
Graphene nanomesh promises extremely efficient in vivo photothermal therapy
, Article Small ; Volume 9, Issue 21 , 2013 , Pages 3593-3601 ; 16136810 (ISSN) ; Ghaderi, E ; Sharif University of Technology
2013
Abstract
Reduced graphene oxide nanomesh (rGONM), as one of the recent structures of graphene with a surprisingly strong near-infrared (NIR) absorption, is used for achieving ultraefficient photothermal therapy. First, by using TiO2 nanoparticles, graphene oxide nanoplatelets (GONPs) are transformed into GONMs through photocatalytic degradation. Then rGONMs functionalized by polyethylene glycol (PEG), arginine-glycine-aspartic acid (RGD)-based peptide, and cyanine 7 (Cy7) are utilized for in vivo tumor targeting and fluorescence imaging of human glioblastoma U87MG tumors having ανβ3 integrin receptors, in mouse models. The rGONM-PEG suspension (1 μg mL -1) exhibits about 4.2- and 22.4-fold higher NIR...
Selective colorimetric detection of pentaerythritol tetranitrate (PETN) using arginine-mediated aggregation of gold nanoparticles
, Article Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy ; Volume 228 , 2020 ; Ghasemi, F ; Hormozi Nezhad, M. R ; Sharif University of Technology
Elsevier B.V
2020
Abstract
Detection of pentaerythritol tetranitrate (PETN) as an explosive has been of great interest because of public safety and military concerns. Here, we have presented a simple, selective and sensitive colorimetric method for direct detection of PETN. The gold nanoparticles (AuNPs) were first exposed to arginine which has primary amines in its structure. Electron deficient –NH2 groups from arginine could strongly interact with –NO2 groups of PETN as electron donors. Hydrogen bonding happens between the –NO2 group of PETN and –NH2 group of arginine molecules. Therefore, selective aggregation of AuNPs happened because of the donor-acceptor and hydrogen bonding interactions. Due to the aggregation,...
Synthesis and Characterization of Dextran Based Magnetic Nanogels as a Gene Delivery Vector and Investigating its Gene Therapy Efficiency
, M.Sc. Thesis Sharif University of Technology ; Madaah Hosseini, Hamid Reza (Supervisor) ; Arefian, Ehsan (Supervisor)
Abstract
The use of magnetic nanoparticles modified with proper surface agent and the ability of controlling by magnetic field, that can induce colloidal stability, is considered as a vector to transfer DNA plasmid, or pDNA in short, in the field of gene therapy. In thiss research, pH-responsive dextran-based magnetic nanogels (dextMNGs) were synthesized via inverse mini-emulsion method. Fourier transformation infrared spectroscopy (FTIR) showed that magnetite nanoparticles (MNPs) were successfully modified with arginine and had amine terminals. FTIR, also, proved that aldehyded dextran was crosslinked by arginine modified magnetite nanoparticles (RMNPs) via pH sensitive imine bonds. X-ray...
Multifunctional core-shell nanoplatforms (gold@graphene oxide) with mediated NIR thermal therapy to promote miRNA delivery
, Article Nanomedicine: Nanotechnology, Biology, and Medicine ; Volume 14, Issue 6 , 2018 , Pages 1891-1903 ; 15499634 (ISSN) ; Akhavan, O ; Adeli, M ; Razzazan, S ; Dinarvand, R ; Zanganeh, S ; Soleimani, M ; Dinarvand, M ; Atyabi, F ; Sharif University of Technology
Elsevier Inc
2018
Abstract
Recent insights into the nanomedicine have revealed that nanoplatforms enhance the efficacy of carrier in therapeutic applications. Here, multifunctional nanoplatforms were utilized in miRNA-101 delivery and NIR thermal therapy to induce apoptosis in breast cancer cells. Au nanorods (NRs) or nanospheres (NSs) covered with graphene oxide (GO) were prepared and functionalized with polyethylene glycol as a stabilizer and poly-L-arginine (P-L-Arg) as a targeting agent. In nanoplatforms, coupling Au@GO prepared stable structures with higher NIR reactivity. P-L-Arg substantially enhanced the cellular uptake and gene retardation of stuffs coated by them. However, rod-shape nanoplatforms indicated...
Nontoxic concentrations of PEGylated graphene nanoribbons for selective cancer cell imaging and photothermal therapy
, Article Journal of Materials Chemistry ; Volume 22, Issue 38 , 2012 , Pages 20626-20633 ; 09599428 (ISSN) ; Ghaderi, E ; Emamy, H ; Sharif University of Technology
2012
Abstract
Reduced graphene oxide nanoribbons functionalized by amphiphilic polyethylene glycol (rGONR-PEG) were applied to attach arginine-glycine-aspartic acid (RGD)-based peptide and cyanine dye 3 (cy3) for targeting ανβ3 integrin receptors on human glioblastoma cell line U87MG and its selective fluorescence imaging, respectively. The rGONR-PEG suspension with a concentration of 100 μg mL -1 showed ∼14 and 2.4-fold higher near infrared (NIR) absorption at 808 nm than GONR (with dimensions of ∼80 nm × 1 μm) and rGO-PEG sheets (with lateral dimensions of ∼2 μm), respectively. The rGONR-PEG-cy3-RGD exhibited highly efficient NIR photothermal therapy performance (concentrations ≥1.0 μg mL-1 resulted in...
Designed amino acid feed in improvement of production and quality targets of a therapeutic monoclonal antibody
, Article PLoS ONE ; Volume 10, Issue 10 , October , 2015 ; 19326203 (ISSN) ; Vaziri, B ; Maleknia, S ; Heydari, A ; Vossoughi, M ; Davami, F ; Mahboudi, F ; Sharif University of Technology
Public Library of Science
2015
Abstract
Cell culture feeds optimization is a critical step in process development of pharmaceutical recombinant protein production. Amino acids are the basic supplements of mammalian cell culture feeds with known effect on their growth promotion and productivity. In this study, we reported the implementation of the Plackett-Burman (PB) multifactorial design to screen the effects of amino acids on the growth promotion and productivity of a Chinese hamster ovary DG-44 (CHO-DG44) cell line producing bevacizumab. After this screening, the amino acid combinations were optimized by the response surface methodology (RSM) to determine the most effective concentration in feeds. Through this strategy, the...
A metabonomics study on crohn's disease using nuclear magnetic resonance spectroscopy
, Article HealthMED ; Volume 6, Issue 11 , July , 2012 , Pages 3577-3583 ; 18402291 (ISSN) ; Kyani, A ; Nejad, M. R ; Rezaye Tavirani, M ; Naderi, N ; Zali, M. R ; Tafazzoli, M ; Oskouie, A. A ; Sharif University of Technology
2012
Abstract
Objective: Crohn's disease (CD) is one the important illnesses can affect any part of the gastrointestinal tract. CD is not easily diagnosed using the clinical tests. Thus, the discovery of proper methods would be a major step towards CD diagnosis. The aim of this study was to seek the metabolic biomarkers causes of CD compare to control group. Materials and Methods: In present study, we employed metabolic profiling using proton nuclear magnetic resonance spectroscopy (1HNMR) to find metabolites in serum which are helpful for the diagnosis of CD. Classification of CD and healthy subject was done using classification and regression trees (CART). The metabolites that caused changes in people...
NMR spectroscopy-based metabolomic study of serum in sulfur mustard exposed patients with lung disease
, Article Biomarkers ; Volume 22, Issue 5 , 2017 , Pages 413-419 ; 1354750X (ISSN) ; Arefi Oskouie, A ; Rezaei Tavirani, M ; Aliannejad, R ; Taheri, S ; Fathi, F ; Naseri, M. T ; Sharif University of Technology
Taylor and Francis Ltd
2017
Abstract
Sulfur mustard (SM) is a vesication chemical warfare agent for which there is currently no antidote. Despite years of research, there is no common consensus about the pathophysiological basis of chronic pulmonary disease caused by this chemical warfare agent. In this study, we combined chemometric techniques with nuclear magnetic resonance (NMR) spectroscopy to explore the metabolic profile of sera from SM-exposed patients. A total of 29 serum samples obtained from 17 SM-injured patients, and 12 healthy controls were analyzed by Random Forest. Increased concentrations of seven amino acids, glycerol, dimethylamine, ketone bodies, lactate, acetate, citrulline and creatine together with the...
Chaperones promote remarkable solubilization of salmonella enterica serovar enteritidis flagellin expressed in escherichia coli
, Article Protein and Peptide Letters ; Volume 27, Issue 3 , 2020 , Pages 210-218 ; Sadeghi, Z ; Tarahomjoo, S ; Yaghmaie, S ; Sharif University of Technology
Bentham Science Publishers
2020
Abstract
Background: Flagellin of Salmonella enterica serovar Enteritidis (SEF) stimulates immune responses to both itself and coapplied antigens. It is therefore used in vaccine development and immunotherapy. Removal of pathogenic S. enterica ser. Enteritidis from SEF production process is advantageous due to the process safety improvement. The protein solubility analysis using SDS-PAGE indicated that 53.49% of SEF expressed in Escherichia coli formed inclusion bodies. However, the protein recovery from inclusion bodies requires a complex process with a low yield. Objective: We thus aim to study possibility of enhancing SEF expression in E. coli in soluble form using chemical and molecular...
Toward visual chiral recognition of amino acids using a wide-range color tonality ratiometric nanoprobe
, Article Analytica Chimica Acta ; Volume 1231 , 2022 ; 00032670 (ISSN) ; Fahimi Kashani, N ; Morad, R ; Jamshidi, Z ; Hormozi Nezhad, M. R ; Sharif University of Technology
Elsevier B.V
2022
Abstract
Chiral recognition has long been a challenging issue to deal with in biological systems, drug design and food authentication. Implementing nanoparticle-based probes with intrinsic or induced chirality in this field has addressed several issues concerning sensitivity, reliability, rapidness and the cost of chiral sensing platforms. Yet, research into chiral nanoprobes that can be used for visual monitoring of chiral substances is still in its infancy. As part of this study, a visual chiral recognition platform has been developed in which a combination of blue-emitting carbon dots (BCDs) and mercaptopropionic acid-capped CdTe quantum dots (MPA-QDs) with inherent chiroptical activity were...
Silica nanoparticle surface chemistry: An important trait affecting cellular biocompatibility in two and three dimensional culture systems
, Article Colloids and Surfaces B: Biointerfaces ; Volume 182 , 2019 ; 09277765 (ISSN) ; Taebnia, N ; Yaghmaei, S ; Shahbazi, M. A ; Mehrali, M ; Dolatshahi Pirouz, A ; Arpanaei, A ; Sharif University of Technology
Elsevier B.V
2019
Abstract
Great advantages bestowed by mesoporous silica nanoparticles (MSNs) including high surface area, tailorable pore diameter and surface chemistry, and large pore volume render them as efficient tools in biomedical applications. Herein, MSNs with different surface chemistries were synthesized and investigated in terms of biocompatibility and their impact on the morphology of bone marrow-derived mesenchymal stem cells both in 2D and 3D culture systems. Bare MSNs (BMSNs) were synthesized by template removing method using tetraethylorthosilicate (TEOS) as a precursor. The as-prepared BMSNs were then used to prepare amine-functionalized (AMSNs), carboxyl-functionalized (CMSNs) and polymeric...
The metabolome profiling of obese and non-obese individuals: Metabolically healthy obese and unhealthy non-obese paradox
, Article Iranian Journal of Basic Medical Sciences ; Volume 23, Issue 2 , 2020 , Pages 186-194 ; Madani, N. H ; Ghoochani, B. F. N. M ; Safari Alighiarloo, N ; Khamseh, M. E ; Sharif University of Technology
Mashhad University of Medical Sciences
2020
Abstract
Objective(s): The molecular basis of “metabolically healthy obese” and “metabolically unhealthy non-obese” phenotypes is not fully understood. Our objective was to identify metabolite patterns differing in obese (metabolically healthy vs unhealthy (MHO vs MUHO)) and non-obese (metabolically healthy vs unhealthy (MHNO vs MUHNO)) individuals. Materials and Methods: This case-control study was performed on 86 subjects stratified into four groups using anthropometric and clinical measurements: MHO (21), MUHO (21), MHNO (22), and MUHNO (22). Serum metabolites were profiled using nuclear magnetic resonance (NMR). Multivariate analysis was applied to uncover discriminant metabolites, and enrichment...