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Comparison of engineered cartilage based on BMSCs and chondrocytes seeded on PVA-PPU scaffold in a sheep model
, Article Journal of Biomedical Materials Research - Part B Applied Biomaterials ; Volume 110, Issue 11 , 2022 , Pages 2411-2421 ; 15524973 (ISSN) ; Sepahdar, A ; Rabiee, N ; Nazbar, A ; Farzad Mohajeri, S ; Dehghan, M. M ; Shokrgozar, M. A ; Majidi, M ; Mardjanmehr, S. H ; Aminianfar, H ; Akbari Javar, H ; Bonakdar, S ; Sharif University of Technology
John Wiley and Sons Inc
2022
Abstract
In this study, polyvinyl alcohol hydrogel chains were crosslinked by polyurethane in order to synthesize a suitable substrate for cartilage lesions. The substrate was fully characterized, and in vitro and in vivo investigations were conducted based on a sheep model. In vitro tests were performed based on the chondrocyte cells with the Alcian Blue and safranin O staining in order to prove the presence of proteoglycan on the surface of the synthesized substrate, which has been secreted by cultures of chondrocytes. Furthermore, the expression of collagen type I, collagen type II, aggrecan, and Sox9 was presented in the chondrocyte cultures on the synthesized substrate through RT-PCR. In...